Archives of Virology

, Volume 164, Issue 1, pp 317–319 | Cite as

The complete genomic sequence of Rhinolophus gammaherpesvirus 1 isolated from a greater horseshoe bat

  • Keita Noguchi
  • Ryusei Kuwata
  • Hiroshi Shimoda
  • Tetsuya Mizutani
  • Eiichi Hondo
  • Ken MaedaEmail author
Annotated Sequence Record


In a comprehensive research project on bat viruses, we successfully isolated a novel herpesvirus from the spleen of a greater horseshoe bat (Rhinolophus ferrumequinum) in Japan using a cell line established from the kidney of the same bat. This herpesvirus was a novel gammaherpesvirus (Rhinolophus gammaherpesvirus 1; RGHV-1), which belonged to the genus Percavirus. The whole RGHV-1 genome (147,790 bp) showed that 12 of the 84 genes predicted to contain open reading frames did not show any homology to those of other herpesviruses.



This study was supported by grants for the Science and Technology Research Partnership for Sustainable Development (SATREPS) project from Japan International Cooperation Agency (JICA) and Japan Agency for Medical Research and Development (AMED).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

A greater horseshoe bat was captured with permission from Yamaguchi Prefecture in 2006. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

705_2018_4040_MOESM1_ESM.pptx (1.4 mb)
Supplementary material 1 (PPTX 1483 kb)
705_2018_4040_MOESM2_ESM.pptx (75 kb)
Supplementary material 2 (PPTX 74 kb)
705_2018_4040_MOESM3_ESM.docx (29 kb)
Supplementary material 3 (DOCX 29 kb)


  1. 1.
    Davison AJ, Eberle R, Ehlers B, Hayward GS et al (2009) The order Herpesvirales. Arch Virol. 154:171–177CrossRefGoogle Scholar
  2. 2.
    Maeda K, Hondo E, Terakawa J, Kiso Y, Nakaichi N et al (2008) Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae). Emerg Infect Dis 14:347–349CrossRefGoogle Scholar
  3. 3.
    Mizutani T, Endoh D, Okamoto M, Shirato K et al (2007) Rapid genome sequencing of RNA viruses. Emerg Infect Dis. 13:322–324CrossRefGoogle Scholar
  4. 4.
    Agius CT, Nagesha HS, Studdert MJ (1992) Equine herpesvirus 5: comparisons with EHV2 (equine cytomegalovirus), cloning, and mapping of a new equine herpesvirus with a novel genome structure. Virology 191:176–186CrossRefGoogle Scholar
  5. 5.
    Hirokawa T, Boon-Chieng S, Mitaku S (1998) SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14:378–379CrossRefGoogle Scholar
  6. 6.
    Lehner PJ, Hoer S, Dodd R, Duncan LM (2005) Downregulation of cell surface receptors by the K3 family of viral and cellular ubiquitin E3 ligases. Immunol Rev. 207:112–125CrossRefGoogle Scholar
  7. 7.
    Wu Z, Ren X, Yang L, Hu Y, Yang J et al (2012) Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces. J Virol. 86:10999–11012CrossRefGoogle Scholar
  8. 8.
    Host KM, Damania B (2016) Discovery of a novel bat gammaherpesvirus. mSphere 1(1):e00016-16Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Keita Noguchi
    • 1
  • Ryusei Kuwata
    • 1
  • Hiroshi Shimoda
    • 1
  • Tetsuya Mizutani
    • 2
  • Eiichi Hondo
    • 3
  • Ken Maeda
    • 1
    Email author
  1. 1.Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary MedicineYamaguchi UniversityYamaguchiJapan
  2. 2.Research and Education Center for Prevention of Global Infectious Diseases of AnimalsTokyo University of Agriculture and TechnologyFuchuJapan
  3. 3.Laboratory of Animal Morphology, Division of Biofunctional Development, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

Personalised recommendations