Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine
A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04.
The complete genome sequence of phage vB_BbrM_PHB04 with annotation has been deposited in GenBank under accession number MF663786.
This study was funded by Research projects of agricultural public welfare industry of China (201403054), and National Science supported planning projects of Hubei Province of China (2014BBB010).
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors. I would like to declare on behalf of my co-authors that we have no conflict of interest.
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