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Complete sequence of a novel alphaendornavirus from the phytopathogenic fungus Arthrocladiella mougeotii

  • Xiaoqin Peng
  • Shexiang Gan
  • Xiaoting Zhang
  • Yingying Zhai
  • Lina Cai
  • Fei Yan
  • Songbai Zhang
Annotated Sequence Record

Abstract

A novel double-stranded RNA (dsRNA) virus, which was provisionally named Arthrocladiella mougeotii endornavirus (AmEV), was isolated from Arthrocladiella mougeotii, the phytopathogenic fungus infecting Lycium chinense in Beijing, China. The genome of AmEV is 11,683 nucleotides in length with a 5′ and 3′ non-coding region of 16 and 50 nt, respectively, as well as a single 11,617-nt long open reading frame potentially encoding a putative protein of 3,871 amino acids with conserved Helicase and RNA-dependent RNA polymerase (RdRp) domains. Phylogenetic analysis based on the the amino acid sequence of the RdRp showed that AmEV is most closely related to Erysiphe cichoracearum endornavirus (EcEV).

Notes

Funding

This work was supported by the National Natural Science Foundation of China (No. 31301638) and the Opening Fund of Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province. We are grateful to Dr. Qingchao Deng at the College of Agriculture, Yangtze University for his help in preparing the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_4028_MOESM1_ESM.pdf (54 kb)
Supplemental Figure 1 Virus detection by RT-PCR (PDF 54 kb)
705_2018_4028_MOESM2_ESM.docx (14 kb)
Supplemental Table 1 Some primers used in the study (DOCX 13 kb)
705_2018_4028_MOESM3_ESM.docx (18 kb)
Supplemental Table 2 Percent amino acid sequence identity of Arthrocladiella mougeotii endornavirus domains compared to those of selected endornaviruses (DOCX 18 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Hubei Collaborative Innovation Center for Grain Industry, Engineering Research Center of Ecology and Agricultural Use of WetlandChina Ministry of Education, Yangtze UniversityJingzhouChina
  2. 2.State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.College of Plant ProtectionHenan Agricultural UniversityZhengzhouChina

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