Archives of Virology

, Volume 164, Issue 1, pp 249–254 | Cite as

Cucurbit aphid-borne yellows virus from melon plants in Brazil is an interspecific recombinant

  • Thiago Marques Costa
  • Rosana Blawid
  • Miguel A. Aranda
  • Débora Maria Sansini Freitas
  • Genira Pereira Andrade
  • Alice Kazuko Inoue-Nagata
  • Tatsuya NagataEmail author
Brief Report


Melon plants with severe yellowing symptoms from in Brazil were analyzed by high-throughput sequencing. Sequences homologous to the genome of the polerovirus cucurbit aphid-borne yellows virus (CABYV) were frequently retrieved. Two draft CABYV genomes were assembled from two pooled melon samples that contained an identical putative recombinant fragment in the 3′ region with an unknown polerovirus. The complete genomes of these isolates revealed by Sanger sequencing share 96.8% nucleotide identity, while both sequences share 73.7% nucleotide identity with a CABYV-N isolate from France. A molecular-clock analysis suggested that CABYV was introduced into Brazil ~ 68 years ago.



This work was supported by CNPq with the project number of 401755/2013-4. MAA work was supported by grant AGL2015-65838 (Ministerio de Economía, Industria y Competitividad, Spain). AKIN and TN are CNPq fellows.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_4024_MOESM1_ESM.doc (37 kb)
Supplementary material 1 (DOC 37 kb)
705_2018_4024_MOESM2_ESM.mp4 (16.5 mb)
Supplementary Figure 1. Animation showing the predicted distribution route of CABYV based on the molecular clock for the P0 gene. (MP4 16932 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Thiago Marques Costa
    • 1
  • Rosana Blawid
    • 1
  • Miguel A. Aranda
    • 2
  • Débora Maria Sansini Freitas
    • 3
  • Genira Pereira Andrade
    • 4
  • Alice Kazuko Inoue-Nagata
    • 5
  • Tatsuya Nagata
    • 1
    Email author
  1. 1.Campus Darcy Ribeiro, Departamento de Biologia CelularUniversidade de BrasíliaBrasíliaBrazil
  2. 2.Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC)MurciaSpain
  3. 3.Embrapa SemiáridoPetrolinaBrazil
  4. 4.Departamento de AgronomiaUniversidade Federal Rural de PernambucoRecifeBrazil
  5. 5.Embrapa HortaliçasBrasíliaBrazil

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