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Archives of Virology

, Volume 163, Issue 11, pp 2985–2995 | Cite as

Acipenser iridovirus-European encodes a replication factor C (RFC) sub-unit

  • Laurane Pallandre
  • Mélanie Lesne
  • Claire de Boisséson
  • François-Xavier Briand
  • Amélie Charrier
  • Thomas Waltzek
  • Patrick Daniel
  • Arthur Tragnan
  • Bastien Debeuf
  • Valérie Chesneau
  • Laurent Bigarré
Original Article

Abstract

New genomic sequence data were acquired for the Acipenser iridovirus-European (AcIV-E), a virus whose complete genome and classification still remain to be elucidated. Here, we obtained the first full-length Major capsid protein (MCP) gene sequence for AcIV-E, as well as two additional open reading frames (ORFs) adjacent to the MCP gene. BLAST searches of the first ORF (α) resulted in no match to any gene or protein in the public databases. The other ORF (β) was identified as a subunit of a replication factor C (RFC), known to function as a clamp loader in eukaryotes, archae and some viruses. The presence of similar RFC genes was confirmed in two distinct, yet related, viruses, the white sturgeon iridovirus and a European variant of Namao virus. The existence of an RFC gene in AcIV-E suggests a genome size larger than that of other classifiable members of the family Iridoviridae along with a mode of replication involving an interaction between a clamp loader and a proliferating nuclear cell antigen. Sequencing and comparison of the full-length RFC gene from various sturgeon samples infected with AcIV-E revealed two distinct clusters of sequences within one particular sample in which the coexistence of two lineages had previously been predicted based on analysis of the partial MCP gene sequence. These genetic data provide further evidence of the circulation of at least two concurrent AcIV-E lineages, sometimes co-infecting cultured European sturgeon.

Notes

Compliance with ethical standards

Funding

This study (ACIVIR2 project) was funded by the European Regional Development Fund (FEDER 160710), the Nouvelle Aquitaine regional council and partner fish farmers.

Conflict of interest

B Debeuf and V. Chesneau work for private companies cited in the authors’ list. The other authors declare that there are no competing interests regarding the publication of this paper.

Ethical approval

The samples originated from moribund farmed fish, not submitted to experimentations and euthanized in accordance with animal welfare and ethics.

Supplementary material

705_2018_3963_MOESM1_ESM.pdf (152 kb)
Supplementary material 1 (PDF 152 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Laurane Pallandre
    • 1
  • Mélanie Lesne
    • 2
  • Claire de Boisséson
    • 3
  • François-Xavier Briand
    • 3
  • Amélie Charrier
    • 2
  • Thomas Waltzek
    • 4
  • Patrick Daniel
    • 2
  • Arthur Tragnan
    • 5
  • Bastien Debeuf
    • 6
  • Valérie Chesneau
    • 7
  • Laurent Bigarré
    • 1
  1. 1.Laboratoire de Ploufragan-PlouzanéANSES, Technopole Brest IroisePlouzanéFrance
  2. 2.Laboratoire des Pyrénées et des LandesMont-de-MarsanFrance
  3. 3.Laboratoire de Ploufragan-PlouzanéANSESPloufraganFrance
  4. 4.Department of Infectious Diseases and Immunology, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  5. 5.Groupement de Défense Sanitaire Aquacole AquitainMont-de-MarsanFrance
  6. 6.SCEA SturgeonSaint-Fort-sur-GirondeFrance
  7. 7.Les Esturgeons de l’AdourRiscleFrance

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