Molecular analysis of Newcastle disease virus isolates reveals a novel XIId subgenotype in Vietnam
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Eight Vietnamese Newcastle disease virus field isolates from 2008–2015 and 3 vaccine specimens were genotyped based on their full F gene sequences and compared to 80 reference strains representing all 18 genotypes. Three isolates formed a novel subgenotype XIId, identified for the first time in Vietnam; while the others clustered as follows: four in subgenotypes VIId and VIIh; two in Genotype I; and two in Genotype II. Evolutionary distance calculations confirmed the Vietnamese XIId isolates were distinct from XIIa and XIIb by 0.062–0.070; and from other genotypes by 0.089–0.245. This data demonstrated that a novel XIId subgenotype emerged in Vietnam indicating considerable genetic diversity, thus highlighting the need to implement antigenic matching during vaccination against NDVs.
This research is funded by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant number 106-NN.02-2013.37. We express our thanks to our provincial veterinary colleagues for helping us in the collection of viral samples during our research. We extend our thanks to Peter Krell of Guelph University (Canada), for the invaluable review of the manuscript.
This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant number 106-NN.02-2013.37.
Compliance with ethical standards
Conflict of interest
The authors declare no competing interests.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Doyle TM (1927) A hitherto unrecorded disease of fowls due to a filter-passing virus. J Comp Pathol Ther 40:144–169Google Scholar
- 4.Diel DG, da Silva LH, Liu H, Wang Z, Miller PJ, Afonso CL (2012) Genetic diversity of avian paramyxovirus type 1: proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infect Genet Evol 12(8):1770–1779. https://doi.org/10.1016/j.meegid.2012.07.012 CrossRefPubMedGoogle Scholar
- 11.Lomniczi B, Wehmann E, Herczeg J, Ballagi-Pordány A, Kaleta EF, Werner O, Meulemans G, Jorgensen PH, Manté AP, Gielkens AL, Capua I, Damoser J (1998) Newcastle disease outbreaks in recent years in western Europe were caused by an old (VI) and a novel genotype (VII). Arch Virol 143(1):49–64. https://doi.org/10.1007/s007050050267 CrossRefPubMedGoogle Scholar
- 12.Maminiaina OF, Gil P, Briand FX, Albina E, Keita D, Andriamanivo HR, Chevalier V, Lancelot R, Martinez D, Rakotondravao R, Rajaonarison JJ, Koko M, Andriantsimahavandy AA, Jestin V, Servan de Almeida R (2010) Newcastle disease virus in Madagascar: identification of an original genotype possibly deriving from a died out ancestor of genotype IV. PLoS One 5(11):e13987. https://doi.org/10.1371/journal.pone.0013987 CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Duan X, Zhang P, Ma J, Chen S, Hao H, Liu H, Fu X, Wu P, Zhang D, Zhang W, Du E, Yang Z (2014) Characterization of genotype IX Newcastle disease virus strains isolated from wild birds in the northern Qinling Mountains, China. Virus Genes 48(1):48–55. https://doi.org/10.1007/s11262-013-0987-y CrossRefPubMedGoogle Scholar
- 15.Chumbe A, Izquierdo-Lara R, Tataje L, Gonzalez R, Cribillero G, González AE, Fernández-Díaz M, Icochea E (2017) Pathotyping and phylogenetic characterization of Newcastle Disease Viruses Isolated in Peru: defining two novel subgenotypes within genotype XII. Avian Dis 61(1):16–24. https://doi.org/10.1637/11456-062016-Reg CrossRefPubMedGoogle Scholar
- 16.Miller PJ, Haddas R, Simanov L, Lublin A, Rehmani SF, Wajid A, Bibi T, Khan TA, Yaqub T, Setiyaningsih S, Afonso CL (2015) Identification of new sub-genotypes of virulent Newcastle disease virus with potential panzootic features. Infect Genet Evol 29:216–229. https://doi.org/10.1016/j.meegid.2014.10.032 CrossRefPubMedGoogle Scholar
- 18.Courtney SC, Susta L, Gomez D, Hines NL, Pedersen JC, Brown CC, Miller PJ, Afonso CL (2013) Highly divergent virulent isolates of Newcastle disease virus from the Dominican Republic are members of a new genotype that may have evolved unnoticed for over 2 decades. J Clin Microbiol 51(2):508–517. https://doi.org/10.1128/JCM.02393-12 CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Snoeck CJ, Owoade AA, Couacy-Hymann E, Alkali BR, Okwen MP, Adeyanju AT, Komoyo GF, Nakouné E, Le Faou A, Muller CP (2013) High genetic diversity of Newcastle disease virus in poultry in West and Central Africa: cocirculation of genotype XIV and newly defined genotypes XVII and XVIII. J Clin Microbiol 51(7):2250–2260. https://doi.org/10.1128/JCM.00684-13 CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Susta L, Jones ME, Cattoli G, Cardenas-Garcia S, Miller PJ, Brown CC, Afonso CL (2015) Pathologic characterization of genotypes XIV and XVII Newcastle disease viruses and efficacy of classical vaccination on specific pathogen-free birds. Vet Pathol 52(1):120–131. https://doi.org/10.1177/0300985814521247 CrossRefPubMedGoogle Scholar
- 21.Xue C, Cong Y, Yin R, Sun Y, Ding C, Yu S, Liu X, Hu S, Qian J, Yuan Q, Yang M, Wang C, Ding Z (2017) Genetic diversity of the genotype VII Newcastle disease virus: identification of a novel VIIj sub-genotype. Virus Genes 53(1):63–70. https://doi.org/10.1007/s11262-016-1404-0 CrossRefPubMedGoogle Scholar
- 25.Bogoyavlenskiy A, Berezin V, Prilipov A, Usachev E, Lyapina O, Korotetskiy I, Zaitceva I, Asanova S, Kydyrmanov A, Daulbaeva K, Shakhvorostova L, Sayatov M, King D (2009) Newcastle disease outbreaks in Kazakhstan and Kyrgyzstan during 1998, 2000, 2001, 2003, 2004, and 2005 were caused by viruses of the genotypes VIIb and VIId. Virus Genes 39(1):94–101. https://doi.org/10.1007/s11262-009-0370-1 CrossRefPubMedGoogle Scholar
- 26.Aldous EW, Mynn JK, Banks J, Alexander DJ (2003) A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathol 32(3):239–256. https://doi.org/10.1080/030794503100009783 CrossRefPubMedGoogle Scholar
- 27.Berhanu A, Ideris A, Omar AR, Bejo MH (2010) Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia. Virol J 7:183. https://doi.org/10.1186/1743-422X-7-183 CrossRefPubMedPubMedCentralGoogle Scholar
- 28.Netter R, Luong NB (1956) [Research on Newcastle disease in Vietnam. I. Isolation of two strains. II. Serological research in various birds. III. Comparison of several vaccines]. Bull Soc Pathol Exot Filiales 49(1):111–118 (French) Google Scholar
- 34.Satharasinghe DA, Murulitharan K, Tan SW, Yeap SK, Munir M, Ideris A, Omar AR (2016) Detection of inter-lineage natural recombination in avian paramyxovirus serotype 1 using simplified deep sequencing platform. Front Microbiol 7:1907. https://doi.org/10.3389/fmicb.2016.01907 CrossRefPubMedPubMedCentralGoogle Scholar