Archives of Virology

, Volume 163, Issue 11, pp 3155–3159 | Cite as

Application of the SureSelect target enrichment system for next-generation sequencing to obtain the complete genome sequence of bovine leukemia virus

  • Tung Duy Dao
  • Vuong Nghia Bui
  • Tsutomu Omatsu
  • Yukie Katayama
  • Tetsuya Mizutani
  • Haruko OgawaEmail author
  • Kunitoshi Imai
Brief Report


In this study, the SureSelect target enrichment system for Illumina Multiplexed Sequencing was applied to proviral DNA sequencing of bovine leukemia virus (BLV). The complete genomic DNA sequences of four Vietnamese BLV strains were successfully obtained with high read depth values and a genome coverage of 100% across all sequenced samples, in less than one week. This study provides the first complete Vietnamese BLV genome sequences. Their genetic variability and phylogenetic relationship were also analyzed and compared with those of 28 whole BLV genome sequences from different parts of the world. The results obtained provided new insights into the genetic diversity of the BLV tax gene, and further enabled us to identify nucleotide mutations in the gene that might not have been detected with the commercial detection kit that is currently available.


Compliance with ethical standards


This study was supported by the Research Project for Improving Food Safety and Animal Health of the Ministry of Agriculture, Forestry and Fisheries of Japan (716057223). This work was partially supported by JSPS KAKENHI Grant Number 15H05260 from the Japan Society for the Promotion of Science, Japan.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The cattle blood samples used in this study were collected by the National Institute of Veterinary Research in accordance with the National Technical Regulation on Animal Diseases–General Requirements for Sample Collection, Storage and Shipment QCVN 01-83:2011/BNNPTNT of Vietnam. All samples were used for the study with the permission of the owners of the cattle farms.

Supplementary material

705_2018_3957_MOESM1_ESM.pdf (227 kb)
Supplementary Figure 1 Alignment of the 5′ LTR sequences (nt 320–430) of four Vietnamese BLV strains (shown in yellow) with FLK-BLV and worldwide BLV strains. Identity with the FLK-BLV strain is indicated by dots. The nucleotide deletions and insertions in Vietnamese BLVs are highlighted in red. The downstream activator sequence (DAS) is indicated in purple (PDF 227 kb)
705_2018_3957_MOESM2_ESM.pdf (847 kb)
Supplementary Figure 2 Alignment of the BLV tax gene sequences of four Vietnamese strains with FLK-BLV and worldwide BLV strains. The FLK-BLV subclone pBLV913 sequence (NCBI, EF600696) was used as a consensus sequence. Identity with the FLK-BLV strain is indicated by dots. The G6VN-BLVs, G10 Myanmar strains, G6 Paraguay strains, and G6 and G10 China strains are indicated in yellow, green, blue and pink, respectively. The seven nucleotide mutations exclusively found in the BLV G6 and G10 strains (highlighted in red) are presented in the figure (PDF 848 kb)
705_2018_3957_MOESM3_ESM.fas (35 kb)
Supplementary material 3 (FAS 35 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Veterinary MedicineObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.National Institute of Veterinary ResearchHanoiVietnam
  3. 3.Research and Education Center for Prevention of Global Infectious Diseases of AnimalsTokyo University of Agriculture and TechnologyTokyoJapan

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