Application of the SureSelect target enrichment system for next-generation sequencing to obtain the complete genome sequence of bovine leukemia virus
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In this study, the SureSelect target enrichment system for Illumina Multiplexed Sequencing was applied to proviral DNA sequencing of bovine leukemia virus (BLV). The complete genomic DNA sequences of four Vietnamese BLV strains were successfully obtained with high read depth values and a genome coverage of 100% across all sequenced samples, in less than one week. This study provides the first complete Vietnamese BLV genome sequences. Their genetic variability and phylogenetic relationship were also analyzed and compared with those of 28 whole BLV genome sequences from different parts of the world. The results obtained provided new insights into the genetic diversity of the BLV tax gene, and further enabled us to identify nucleotide mutations in the gene that might not have been detected with the commercial detection kit that is currently available.
Compliance with ethical standards
This study was supported by the Research Project for Improving Food Safety and Animal Health of the Ministry of Agriculture, Forestry and Fisheries of Japan (716057223). This work was partially supported by JSPS KAKENHI Grant Number 15H05260 from the Japan Society for the Promotion of Science, Japan.
Conflict of interest
The authors declare that they have no conflict of interest.
The cattle blood samples used in this study were collected by the National Institute of Veterinary Research in accordance with the National Technical Regulation on Animal Diseases–General Requirements for Sample Collection, Storage and Shipment QCVN 01-83:2011/BNNPTNT of Vietnam. All samples were used for the study with the permission of the owners of the cattle farms.
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