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Archives of Virology

, Volume 163, Issue 11, pp 3185–3189 | Cite as

Molecular characterization of a new gammapartitivirus isolated from the citrus-pathogenic fungus Penicillium digitatum

  • Zhu Yang
  • Hui Geng
  • Yongliang Zheng
  • Yongze Yuan
  • Menglan Wang
  • Jiali Mao
  • Tingfu Zhang
  • Yuhui Niu
  • Deli Liu
Annotated Sequence Record

Abstract

To date, partitiviruses, including gammapartitiviruses, have been extensively studied in various fungal hosts but have not been reported in Penicillium digitatum (also called green mold, the pathogenic fungus infecting citrus). In the present work, we isolated and molecularly characterized a double-stranded RNA (dsRNA) partitivirus from citrus green mold, which we have named “Penicillium digitatum gammapartitivirus 1” (PdGV1). The bisegmented genome of PdGV1 contains two dsRNA segments (dsRNA1 and dsRNA2) with a length of 1795 bp and 1622 bp, respectively. Each of the two genomic dsRNAs contains a single open reading frame encoding a putative RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. Phylogenetic analysis based on RdRp and CP sequences showed that PdGV1 clustered with mycoviruses belonging to the genus Gammapartitivirus, family Partitiviridae, e.g., Penicillium stoloniferum virus S. The 5’- and 3’-untranslated regions (UTRs) of the PdGV1 genomic dsRNAs both contained unique conserved RNA motifs that have never been found in any other partitivirus. This is the first report of a new gammapartitivirus that infects the citrus-pathogenic fungus P. digitatum.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.31371893 and 31071653), the Self-Determined Research Funds of CCNU from the Colleges’ Basic Research and Operation of MOE (No. CCNU17CG003), and the Open Funding from Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (No. 2017BW03). We sincerely thank Dr. Huazhong Shi (Department of Chemistry and Biochemistry, Texas Tech University, USA) for English language editing.

Funding

This work was financially supported by the National Natural Science Foundations of China (No. 31371893 and 31071653), the Self-Determined Research Funds of CCNU from the Colleges’ Basic Research and Operation of MOE (No. CCNU17CG003), and the Open Funding from Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (No. 2017BW03).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3951_MOESM1_ESM.pdf (109 kb)
Supplementary material 1 (PDF 108 kb)

References

  1. 1.
    Smilanick JL, Mansour MF, Mlikota Gabler F, Goodwine WR (2006) The effectiveness of pyrimethanil to inhibit germination of Penicillium digitatum and to control citrus green mold after harvest. Postharvest Biol Technol 4:75–85CrossRefGoogle Scholar
  2. 2.
    Macarisin D, Cohen L, Eick A, Rafael G, Belausov E, Wisniewski M, Droby S (2007) Penicillium digitatum suppresses production of hydrogen peroxide in host tissue during infection of citrus fruit. Phytopathol 97:1491–1500CrossRefGoogle Scholar
  3. 3.
    Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368CrossRefPubMedGoogle Scholar
  4. 4.
    Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci 107:8387–8392CrossRefPubMedGoogle Scholar
  5. 5.
    Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384CrossRefGoogle Scholar
  6. 6.
    Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462–463:227–235CrossRefPubMedGoogle Scholar
  7. 7.
    Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141CrossRefPubMedGoogle Scholar
  8. 8.
    Niu Y, Zhang T, Zhu Y, Yuan Y, Wang S, Liu J, Liu D (2016) Isolation and characterization of a novel mycovirus from Penicillium digitatum. Virology 494:15–22CrossRefPubMedGoogle Scholar
  9. 9.
    Niu YH, Yuan YZ, Mao JL, Yang Z, Cao QW, Zhang TF, Wang SQ, Liu DL (2018) Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis. Scient Rep 8:5513CrossRefGoogle Scholar
  10. 10.
    Pandey B, Naidu RA, Grove GG (2018) Detection and analysis of mycovirus—related RNA viruses from grape powdery mildew fungus Erysiphe necator. Arch Virol 163:1019–1030CrossRefPubMedGoogle Scholar
  11. 11.
    Sotaro C, Lakha S, Lin YH, Sasaki A, Satoko K, Suzuki N (2009) A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 24:12801–12812Google Scholar
  12. 12.
    Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403CrossRefPubMedGoogle Scholar
  13. 13.
    Coutts RHA, Livieratos IC (2003) A rapid method for sequencing the 5’- and 3’-termini of dsRNA viral templates using RLM-RACE. J Phytopathol 151:525–527CrossRefGoogle Scholar
  14. 14.
    Thapa V, Turner GG, Hafenstein S, Overton BE, Vanderwolf KJ, Roossinck MJ (2016) Using a novel partitivirus in Pseudogymnoascus destructans to understand the epidemiology of White-nose syndrome. PLoS Pathog 12(12):e1006076CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Yu JX, Zhu JZ, Wang Y, Zhang CJ, Zhong J, Zhu HJ, Gao BD, Zhou Q (2018) Molecular characterization of a putative gammapartitivirus in the phytopathogenic fungus Nigrospora oryzae. Arch Virol 163:1091–1095CrossRefPubMedGoogle Scholar
  16. 16.
    Kim JW, Kim SY, Kim KM (2003) Genome organization and expression of Penicillium stoloniferum virus S. Virus Genes 27:249–256CrossRefPubMedGoogle Scholar
  17. 17.
    Hacker CV, Brasier CM, Buck KW (2006) Determination of the 5’- and 3’-terminal sequences completes the sequences of the two double-stranded RNAs of Penicillium stoloniferum virus S. Virus Genes 32:137–138CrossRefPubMedGoogle Scholar
  18. 18.
    Leticia B, Tero TT, Jarkko H, Julio JD, Libor J (2015) The European race of Gremmeniella abietina hosts a single species of Gammapartitivirus showing a global distribution and possible recombinant events in its history. Fungal Biol 119:125–135CrossRefGoogle Scholar
  19. 19.
    Cañizares MC, Pérez-Artés E, García-Pedrajas NE, García-Pedrajas MD (2015) Characterization of a new partitivirus strain in Verticillium dahlia provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions. Phytopathol Mediterr 54:516–523Google Scholar
  20. 20.
    Rong R, Rao S, Scott SW, Carner GR, Tainter FH (2002) Complete sequence of the genome of two dsRNA viruses from Discula destructiva. Virus Res 90:217–224CrossRefPubMedGoogle Scholar
  21. 21.
    Bhatti MF, Bignell EM, Coutts RHA (2011) Complete nucleotide sequences of two dsRNAs associated with a new partitivirus infecting Aspergillus fumigatus. Arch Virol 156:1677–1680CrossRefPubMedGoogle Scholar
  22. 22.
    Crawford LJ, Osman TAM, Coutts RHA, Buck KW (2006) Molecular characterization of a partitivirus from Ophiostoma himal-ulmi. Virus Genes 33:33–39CrossRefPubMedGoogle Scholar
  23. 23.
    Nogawa M, Kgeyama T, Nakatani A, Taguchi G, Shimosaka M (1996) Cloning and characterization of mycovirus double-straned RNA from the plant pathogenic fungus, Fusarium solani f. sp. Robiniae +. Biosci Biotech Biochem 60:784–788CrossRefGoogle Scholar
  24. 24.
    Liu WX, Duns G, Chen JS (2008) Genomic characterization of a novel partitivirus infecting Aspergillus ochraceus. Virus Genes 37:322–327CrossRefPubMedGoogle Scholar
  25. 25.
    Svanella-Dumas L, Theil S, Barret M, Candresse T (2018) Complete genomic sequence of Raphanus sativus cryptic virus 4 (RsCV4), a novel alphapartitivirus from radish. Arch Virol 163:1097–1100CrossRefPubMedGoogle Scholar
  26. 26.
    Zhong J, Chen D, Lei X, Zhu H, Zhu J, Gao B (2014) Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001. Virus Res 190:104–109CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Zhu Yang
    • 1
  • Hui Geng
    • 1
  • Yongliang Zheng
    • 2
  • Yongze Yuan
    • 1
  • Menglan Wang
    • 1
  • Jiali Mao
    • 1
  • Tingfu Zhang
    • 1
  • Yuhui Niu
    • 1
  • Deli Liu
    • 1
  1. 1.Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life SciencesCentral China Normal UniversityWuhanPeople’s Republic of China
  2. 2.College of Life SciencesHuanggang Normal UniversityHuanggangPeople’s Republic of China

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