Sequencing, genome analysis and host range of a novel Ralstonia phage, RsoP1EGY, isolated in Egypt

  • Abdelmonim Ali Ahmad
  • Kamel M. Elhalag
  • Hardian Susilo Addy
  • Mohamed A. Nasr-Eldin
  • Ahmed S. Hussien
  • Qi Huang
Annotated Sequence Record

Abstract

A novel Ralstonia phage was isolated from soil in Egypt. It was designated Ralstonia phage RsoP1EGY using our phage identifier naming approach to reflect the phage’s bacterial host species, characteristics and origin. When tested, this phage specifically infected only race 3 biovar 2 phylotype IIB sequevar 1, and not non-race 3 biovar 2 strains of Ralstonia solanacearum. The phage has an icosahedral capsid of 60 ± 5 nm in diameter with a short tail of 15 ± 5 nm in length, typical of a podovirus. The genome of RsoP1EGY is 41,297 bp in size, containing 50 open reading frames, with no significant sequence identity to any other reported R. solanacearum or non-Ralstonia phages, except to the recently deposited but unreported and unclassified Ralstonia phage DU_RP_I. RsoP1EGY is the first sequenced and characterized R. solanacearum phage isolated in Egypt.

Notes

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3844_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© This is a U.S. government work and its text is not subject to copyright protection in the United States; however, its text may be subject to foreign copyright protection 2018

Authors and Affiliations

  • Abdelmonim Ali Ahmad
    • 1
    • 2
  • Kamel M. Elhalag
    • 3
    • 4
  • Hardian Susilo Addy
    • 1
    • 5
  • Mohamed A. Nasr-Eldin
    • 6
  • Ahmed S. Hussien
    • 3
    • 7
  • Qi Huang
    • 1
  1. 1.Floral and Nursery Plants Research UnitUnited States National Arboretum, Agricultural Research Service, United States Department of AgricultureBeltsvilleUSA
  2. 2.Department of Plant Pathology, Faculty of AgricultureMinia UniversityEl-miniaEgypt
  3. 3.Bacterial Diseases Research Department, Plant Pathology Research InstituteAgricultural Research Center (ARC)GizaEgypt
  4. 4.Potato Brown Rot Project, Ministry of AgricultureGizaEgypt
  5. 5.Department of Plant Protection, Faculty of AgricultureUniversity of JemberJemberIndonesia
  6. 6.Botany Department, Faculty of ScienceBenha UniversityBenhaEgypt
  7. 7.Central Administration of Plant QuarantineGizaEgypt

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