Sequence analysis and genomic organization of a new insect iflavirus, Sogatella furcifera honeydew virus 1

  • Nan Wu
  • Peipei Zhang
  • Wenwen Liu
  • Mengji Cao
  • Xifeng Wang
Annotated Sequence Record

Abstract

A novel iflavirus, tentatively named “Sogatella furcifera honeydew virus 1” (SFHV1), discovered through transcriptome sequencing analysis of white-backed planthoppers (Sogatella furcifera) collected in southern China, is described here. The full genome of SFHV1 is 10,837 nucleotides (nt) long, including the polyA tail, and shares 65.5% and 64.5% genomic identity with Laodelphax striatellus picorna-like virus 2 and Laodelphax striatella honeydew virus 1, respectively. On the basis of the phylogenetic analysis of the complete genomic sequence and the deduced RdRp amino acid sequence of SFHV1 with other iflaviruses, we suggest that it is a member of a new species in the genus Iflavirus, family Iflaviridae.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

There was no experimental work with humans in this study.

Supplementary material

705_2018_3817_MOESM1_ESM.ppt (2.3 mb)
Supplementary material 1 (PPT 2343 kb)
705_2018_3817_MOESM2_ESM.doc (54 kb)
Supplementary material 2 (DOC 53 kb)
705_2018_3817_MOESM3_ESM.docx (16 kb)
Supplementary material 3 (DOCX 15 kb)

References

  1. 1.
    Flanegan JB, Petterson RF, Ambros V, Hewlett NJ, Baltimore D (1977) Covalent linkage of a protein to a defined nucleotide sequence at the 5’-terminus of virion and replicative intermediate RNAs of poliovirus. Proc Natl Acad Sci USA 74(3):961–965CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Valles SM, Chen Y, Firth AE, Dma G, Hashimoto Y, Herrero S, de Miranda JR, Ryabov E, Consortium IR (2017) ICTV Virus Taxonomy Profile: Iflaviridae. J Gen Virol 98(4):527–528CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Oers MMV (2010) Genomics and Biology of Iflaviruses. Insect Virology. Caister Academic Press, Norfolk, pp 231–250Google Scholar
  4. 4.
    Shen M, Cui L, Ostiguy N, Coxfoster D (2005) Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee host and the parasitic varroa mite. J Gen Virol 86(8):2281–2289CrossRefPubMedGoogle Scholar
  5. 5.
    Genersch E, Yue C, Fries I, de Miranda JR (2006) Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities. J Invertebr Pathol 91(1):61–63CrossRefPubMedGoogle Scholar
  6. 6.
    Hashimoto Y, Kawase S (1983) Characteristics of structural proteins of infectious flacherie virus from the silkworm, Bombyx mori. J Invertebr Pathol 41(1):68–76CrossRefGoogle Scholar
  7. 7.
    Zhang P, Liu Y, Liu W, Cao M, Massart S, Wang X (2017) Identification, characterization and full-length sequence analysis of a novel polerovirus associated with wheat leaf yellowing disease. Front Microbiol 8(9):1689CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Nikolaj B, Jan H, Søren B, Dieter B (1996) Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks. Protein Sci 5(11):2203–2216CrossRefGoogle Scholar
  9. 9.
    Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol 28(5):375–430CrossRefGoogle Scholar
  10. 10.
    Gorbalenya AE, Koonin EV (1989) Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acids Res 17(21):8413–8440CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Gorbalenya AE, Donchenko AP, Blinov VM, Koonin EV (1989) Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold. FEBS Lett 243(2):103–114CrossRefPubMedGoogle Scholar
  12. 12.
    Ongus JR, Roode EC, Pleij CW, Vlak JM, van Oers MM (2006) The 5’ non-translated region of varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells. J Gen Virol 87(11):3397–3407CrossRefPubMedGoogle Scholar
  13. 13.
    Wu TY, Wu CY, Chen YJ, Chen CY, Wang CH (2007) The 5’ untranslated region of Perina nuda virus (PnV) possesses a strong internal translation activity in baculovirus-infected insect cells. FEBS Lett 581(16):3120–3126CrossRefPubMedGoogle Scholar
  14. 14.
    Lee YF, Nomoto A, Detjen BM, Wimmer E (1977) The genome-linked protein of picornaviruses: I. A protein covalently linked to poliovirus genome RNA. Proc Natl Acad Sci USA 74(1):59–63CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  2. 2.National Citrus Engineering Research Center, Citrus Research InstituteSouthwest UniversityChongqingChina

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