Archives of Virology

, Volume 163, Issue 5, pp 1383–1386 | Cite as

The first complete genome sequence of narcissus latent virus from Narcissus

  • Fangluan Gao
  • Jianguo Shen
  • Furong Liao
  • Wei Cai
  • Shuangqing Lin
  • Hongkai Yang
  • Shouling Chen
Annotated Sequence Record

Abstract

The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge, this is the first report of the complete sequence of a NLV isolate.

Notes

Acknowledgements

We thank Dr Fengping Chen (Fujian Agriculture and Forestry University), Dr Sally Potter (The Australian National University) and two anonymous reviewers for comments and suggestions about the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3741_MOESM1_ESM.pdf (443 kb)
A radar chart for comparison of the sequence identity of Narv-NL with two macluraviruses. Pairwise identities are indicated in colour solid and dashed lines. The thresholds for species demarcation for the family Potyviridae are indicated with black lines (PDF 443 kb)
705_2018_3741_MOESM2_ESM.pdf (51 kb)
Supplementary material 2 (PDF 50 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Fujian Key Laboratory of Plant Virology, Institute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  2. 2.Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Inspection and Quarantine Technology CenterFujian Exit-Entry Inspection and Quarantine BureauFuzhouPeople’s Republic of China
  3. 3.Inspection and Quarantine Technology CenterXiamen Exit-Entry Inspection and Quarantine BureauXiamenPeople’s Republic of China

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