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Complete genome sequence of a novel mitovirus from the wheat stripe rust fungus Puccinia striiformis

  • Li Zheng
  • Jing Zhao
  • Xiaofei Liang
  • Hua Zhuang
  • Tuo Qi
  • Zhensheng KangEmail author
Annotated Sequence Record

Abstract

The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis indicated that a single large open reading frame (ORF) is present on the positive strand when the fungal mitochondrial genetic code is used. The single ORF encodes a putative RNA-dependent RNA polymerase of 743 amino acids with a molecular mass of 84.9 kDa that shares the closest similarity with the corresponding proteins of Cronartium ribicola mitovirus 5 and Helicobasidium mompa mitovirus 1-18 (34% and 35% aa sequence identity, respectively). Phylogenetic analysis further indicated that PsMV1 is a new member of the genus Mitovirus within the family Narnaviridae. This is the first report of the full-length nucleotide sequence of a novel mitovirus, PsMV1, from the causal agent of wheat stripe rust.

Notes

Funding

This study was financially supported by the National Natural Science Foundation of China (31701732).

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with animals or human participants performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Li Zheng
    • 1
    • 2
  • Jing Zhao
    • 1
  • Xiaofei Liang
    • 1
  • Hua Zhuang
    • 1
  • Tuo Qi
    • 1
  • Zhensheng Kang
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant ProtectionNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Institute of Tropical Agriculture and Forestry, Hainan University, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of EducationHaikouPeople’s Republic of China

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