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Genomic sequencing of a virus representing a novel type within the species Dyopipapillomavirus 1 in an Indian River Lagoon bottlenose dolphin

  • Galaxia Cortés-Hinojosa
  • Kuttichantran Subramaniam
  • James F. X. WellehanJr.
  • Terry Fei Fan Ng
  • Eric Delwart
  • Stephen D. McCulloch
  • Juli D. Goldstein
  • Adam M. Schaefer
  • Patricia A. Fair
  • John S. Reif
  • Gregory D. Bossart
  • Thomas B. WaltzekEmail author
Original Article

Abstract

Fecal samples collected from free-ranging Atlantic bottlenose dolphins (BDs) in the Indian River Lagoon of Florida were processed for viral discovery using a next-generation sequencing (NGS) approach. A 693-bp contig identified in the NGS data was nearly identical to the partial L1 gene sequence of a papillomavirus (PV) previously found in a penile papilloma in a killer whale (Orcinus orca). Based on this partial bottlenose dolphin papillomavirus (BDPV) sequence, a nested inverse PCR and primer-walking strategy was employed to generate the complete genome sequence. The full BDPV genome consisted of 7299 bp and displayed a typical PV genome organization. The BDPV E6 protein contained a PDZ-binding motif, which has been shown to be involved in carcinogenic transformation involving high-risk genital human PVs. Screening of 12 individual fecal samples using a specific endpoint PCR assay revealed that the feces from a single female BD displaying a genital papilloma was positive for the BDPV. Genetic analysis indicated that this BDPV (Tursiops truncatus papillomavirus 8; TtPV8) is a new type of Dyopipapillomavirus 1, previously sequenced from an isolate obtained from a penile papilloma in a harbor porpoise (Phocoena phocoena). Although only a partial L1 sequence has been determined for a PV detected in a killer whale genital papilloma, our finding of a nearly identical sequence in an Atlantic BD may indicate that members of this viral species are capable of host jumping. Future work is needed to determine if this virus is a high-risk PV that is capable of inducing carcinogenic transformation and whether it poses a significant health risk to wild delphinid populations.

Notes

Funding

This study was partially funded by Florida Atlantic University (award no. 00086229), the Georgia Aquarium (award no. 03184), and the Florida Fish and Wildlife Conservation Commission (award no. 00126905).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee at the University of Florida (IACUC Protocol no. 201508900). BD samples used in this study were collected under US National Marine Fisheries Service Scientific Research Permit no. 14352.

Supplementary material

705_2018_4117_MOESM1_ESM.pdf (823 kb)
Fig. S1 Genetic comparison of the L1 gene of a novel type of Dyopipapillomavirus 1 from a bottlenose dolphin (Tursiops truncatus papillomavirus 8; TtPV8) to those of representatives of 49 accepted type species in the family Papillomaviridae. Values are expressed as percent nucleotide sequence identity. See Table S3 for PV abbreviations. (PDF 823 kb)
705_2018_4117_MOESM2_ESM.doc (56 kb)
Supplementary material 2 (DOC 55 kb)
705_2018_4117_MOESM3_ESM.doc (54 kb)
Supplementary material 3 (DOC 54 kb)
705_2018_4117_MOESM4_ESM.doc (112 kb)
Supplementary material 4 (DOC 112 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Small Animal Clinical Sciences, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  2. 2.Department of Infectious Diseases and Immunology, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  3. 3.Department of Comparative, Diagnostic and Population Medicine, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  4. 4.College of Veterinary MedicineUniversity of GeorgiaAthensUSA
  5. 5.Blood Systems Research InstituteSan FranciscoUSA
  6. 6.Department of Laboratory MedicineUniversity of California at San FranciscoSan FranciscoUSA
  7. 7.Division of Marine Mammal Research and Conservation, Center of Marine Ecosystems Health, Harbor Branch Oceanographic InstitutionFlorida Atlantic UniversityFort PierceUSA
  8. 8.Protect Wild Dolphins AllianceVero BeachUSA
  9. 9.National Oceanic and Atmospheric Administration, National Ocean ServiceCenter for Coastal Environmental Health and Biomolecular ResearchCharlestonUSA
  10. 10.Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical SciencesColorado State UniversityFort CollinsUSA
  11. 11.Georgia AquariumAtlantaUSA
  12. 12.Division of Comparative Pathology, Miller School of MedicineUniversity of MiamiMiamiUSA

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