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First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes

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Abstract

Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population.

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Acknowledgments

The authors wish to thank Mónica Florin (INTA) and Matías Ottaviani (INTA) for help in drafting the manuscript and figures. This work was supported by Fonds de la Recherche Scientifique (FRS-FNRS) and CONICET. SM is a fellow of the CONICET (Argentina).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Instituto de Virología, Centro de Investigaciones en Ciencias veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25, Castelar, B1712WAA, Buenos Aires, Argentina

    Silvina S. Maidana, María I. Craig, Osvaldo Zabal & Sonia A. Romera

  2. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, C1033AAJ, Ciudad Autónoma de Buenos Aires, Argentina

    Silvina S. Maidana, María I. Craig & Sonia A. Romera

  3. Cátedra de Inmunogenética, Facultad de Ciencias exactas, Químicas y Naturales, Universidad de Morón, Cabildo 134, Morón, B1708JPD, Buenos Aires, Argentina

    Silvina S. Maidana & Sonia A. Romera

  4. Cátedra de Teriogenologia, Facultad de Veterinaria, Universidad Nacional del Nordeste, 25 de Mayo 868, 3400, Corrientes, Argentina

    José L. Konrad & Gustavo Crudeli

  5. Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research on Animal Health center and Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, 20, B43b, 4000, Liège, Belgium

    Axel Mauroy & Etienne Thiry

  6. Cátedra de Inmunología, Universidad del Salvador, Champagnat 1599-Ruta Panamericana-Km 54.5, B1630AHU, Pilar, Provincia de Buenos Aires, Argentina

    Sonia A. Romera

Authors
  1. Silvina S. Maidana
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  2. José L. Konrad
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  3. María I. Craig
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  4. Osvaldo Zabal
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  5. Axel Mauroy
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  6. Etienne Thiry
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  7. Gustavo Crudeli
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  8. Sonia A. Romera
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Correspondence to Silvina S. Maidana.

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Maidana, S.S., Konrad, J.L., Craig, M.I. et al. First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes. Arch Virol 159, 2917–2923 (2014). https://doi.org/10.1007/s00705-014-2146-8

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  • DOI: https://doi.org/10.1007/s00705-014-2146-8

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