Tyrosine-hydroxylase immunoreactivity in the mouse transparent brain and adrenal glands

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Working on catecholamine systems for years, the neuropharmacologist Arvid Carlsson has made a number of important and pioneering discoveries, which have highlighted the key role of these neuronal and peripheral neurotransmitters in brain functions and adrenal regulations. Since then, major advances have been made concerning the distribution of the catecholaminergic systems in particular by studying their rate-limiting enzyme, tyrosine hydroxylase (TH). Recently new methods of tissue transparency coupled with in toto immununostaining and three-dimensional (3D) imaging technologies allow to precisely map TH immunoreactive pathways in the mouse brain and adrenal glands. High magnification images and movies obtained with combined technologies (iDISCO+ and light-sheet microscopy) are presented in this review dedicated to the pioneer work of Arvid Carlsson and his collaborators.

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  • 17 November 2018

    Unfortunately, the given name and family name of the fourth author was incorrectly tagged in the xml data, therefore it is abbreviated wrongly as ‘‘Goazigo AR’’ in Pubmed. The correct given name is Annabelle and family name is Reaux‑Le Goazigo.


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The present study was supported by Sorbonne and Normandie Universities, the Institut National de la Santé et de la Recherche Médicale (INSERM) and the Association Française d’Epargne et de Retraite (AFER). Images were obtained on PRIMACEN (, the Cell Imaging Platform of Normandy, IRIB, Faculty of Sciences, University of Rouen, 76821 Mont-Saint-Aignan.

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Correspondence to William Rostène.

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Godefroy, D., Rostène, W., Anouar, Y. et al. Tyrosine-hydroxylase immunoreactivity in the mouse transparent brain and adrenal glands. J Neural Transm 126, 367–375 (2019) doi:10.1007/s00702-018-1925-x

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  • Tyrosine hydroxylase
  • Dopamine mapping
  • Mouse brain
  • Adrenals
  • Clearing
  • iDISCO+