Plant Systematics and Evolution

, Volume 305, Issue 7, pp 487–502 | Cite as

Multi-locus phylogenetic reconstructions reveal ample reticulate relationships among genera in Anthemideae subtribe Handeliinae (Compositae)

  • Christoph OberprielerEmail author
  • Hajar Hassanpour
  • Ali Sonboli
  • Tankred Ott
  • Florian Wagner
Original Article


In order to clarify the phylogenetic relationships among the genera of Compositae-Anthemideae subtribe Handeliinae, we analysed sequence variation on both the plastidic (cpDNA trnL-rpl32 intergenic spacer region) and the nuclear genome (nrDNA ITS + 5.8S + ETS and three single-copy markers) for representatives of all genera assigned to this subtribe in preceding morphology- and molecular-based analyses. Incongruence among the individual gene trees reconstructed is interpreted as being due to extensive hybridisation between the evolutionary lineages because a coalescent-based species-tree reconstruction, that takes gene-tree incongruence due to incomplete lineage sorting into account, leads to a very limited number of monophyletic groups with statistically significant support values. Nevertheless, our present analyses demonstrate (a) the close relationship of subtribes Handeliinae and Artemisiinae, (b) the lack of phylogenetic independence of subtribes Handeliinae and Cancriniinae, (c) the inclusion of the genus Microcephala in the Handeliinae (as sister to Cancrinia), (d) the close relationship of Lepidolopsis and Tanacetopsis, and (e) the subdivision of Allardia into two lineages, with A. subg. Allardia being related to Trichanthemis and Ugamia (all of them with hairy achenes) and A. subg. Waldheimia to the genus Richteria (glabrous pericarp).


Asteraceae Gene trees Next-generation sequencing Species tree Subtribes Taxonomy 



We would like to express our sincerest thanks to Nicole Schmelzer, the technician in the molecular laboratory of the Evolutionary and Systematic Botany Group at Regensburg University. The curators of herbaria B, FUMH, M/MSB, S, and W are thanked for allowing us to extract DNA from herbarium specimens housed in their collections. Comments of two anonymous reviewers are thankfully acknowledged and have considerably improved the present contribution.


This work was financially supported by the research council of the University of Tarbiat Modares through a PhD student fellowship grant to HH.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interests.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Evolutionary and Systematic Botany GroupInstitute of Plant Sciences, University of RegensburgRegensburgGermany
  2. 2.Department of Plant Biology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran

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