Evidence for fungal sequence contamination in plant transcriptome databases
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Sequences aligning partially with plant arginyl-tRNA synthetase but that showed unexpected mitochondrial characteristics, which do not occur in plants, have been detected in the plant transcriptome database. BLAST screening of this database with vertebrate nuclear-encoded mitochondrial arginyl-tRNA synthetase revealed a set of 23 sequences that on further analysis showed high similarity to fungal genomic DNA. A detailed knowledge of the gene organization of plant arginyl-tRNA synthetase and being aware of the mitochondrial origin of fungal cytoplasmic arginyl-tRNA synthetases have provided convincing evidence that the NCBI plant transcriptome (TSA) database is contaminated with data from fungal material.
KeywordsArginyl-tRNA synthetase Database contamination Fungal RNA Mitochondrial gene Plant transcriptome
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The author declares that he has no conflict of interest.
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