Microchimica Acta

, 187:7 | Cite as

A composite prepared from MnO2 nanosheets and a deep eutectic solvent as an oxidase mimic for the colorimetric determination of DNA

  • Jing Chen
  • Yuzhi WangEmail author
  • Xiaoxiao Wei
  • Rui Ni
  • Jiaojiao Meng
  • Fangting Xu
  • Ziwei Liu
Original Paper


A composite was fabricated from deep eutectic solvent and MnO2 nanosheets (DES/MnO2) and is shown to be a viable oxidase mimic. The property, morphology and composition of DES/MnO2 was characterized. DES/MnO2 displays oxidase-like activity and can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue product (oxTMB) with an absorption maximum at 652 nm. Due to the presence of the DES, the polyanionic and negatively charged DNA is easily adsorbed on the surface of the composite by hydrogen bonding and electrostatic interactions. This leads to the inhibition of the oxidase-mimicking activity of DES/MnO2. This finding was used to design a colorimetric method for the determination of DNA. The assay work in the 10–100 μg mL−1 DNA concentration range and has a detection limit of 0.37 μg mL−1. The inhibiting mechanism was further studied by zeta potential measurements, dynamic light scattering and transmission electron microscopy. The selectivity study shows the DES/MnO2-TMB system to be highly selective for DNA when compared with many proteins, carbohydrates, salts and amino acid. RNA, on the other hand, interferes. The real sample analysis result illustrates that the new method can be used for the detection of DNA in bovine whole blood.

Graphical abstract

A novel oxidase mimic based on deep eutectic solvent-functionalized MnO2 nanosheets was synthesized, which can directly catalyze oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB, colorless) to oxTMB (blue). A sensitive and convenient colorimetric strategy for visual detection of DNA was established through DES/MnO2-TMB sensing system.


Sensing Adsorption Modulator 3,3′,5,5′-Tetramethylbenzidine Inhibition Bovine whole blood 



The authors greatly appreciate the financial supports by the National Natural Science Foundation of China (No.21675048) and Major Industrial Support Programs of Guizhou Province QKHZC[2019]2959.

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

604_2019_4021_MOESM1_ESM.docx (8.7 mb)
ESM 1 (DOCX 8.67 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Jing Chen
    • 1
    • 2
  • Yuzhi Wang
    • 1
    Email author
  • Xiaoxiao Wei
    • 1
  • Rui Ni
    • 1
  • Jiaojiao Meng
    • 1
  • Fangting Xu
    • 1
  • Ziwei Liu
    • 1
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityChangshaPeople’s Republic of China
  2. 2.College of Material and Chemical EngineeringTongren UniversityTongrenPeople’s Republic of China

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