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Microchimica Acta

, 187:4 | Cite as

DNA-templated copper nanoparticles as signalling probe for electrochemical determination of microRNA-222

  • Ya Wang
  • Wenwen Meng
  • Xi Chen
  • Yuzhong ZhangEmail author
Original Paper
  • 33 Downloads

Abstract

An ultrasensitive electrochemical biosensor is described for the determination of microRNAs. It is based on the use of DNA-templated copper nanoparticles (Cu NPs) as signalling probe. MicroRNA-222 was selected as the model analyte. The probe was obtained from two different oligonucleotides (containing complementary bases) via hybridization chain reaction to form long DNA concatemers as template. The Cu NPs were formed by reaction of ascorbate with copper sulfate. The biosensor was fabricated as follows: (a) Capture probe (cDNA) with a thiolated group was immobilized on reduced graphene oxide modified with gold nanoparticles (rGO/Au NPs), (b) materials was placed on a glassy carbon electrode (GCE); (c) the modified electrode (cDNA/rGO/Au NPs/GCE) was sequentially hybridized with microRNA-222 and signal probe; this results in the formation of a sandwich structure of cDNA-microRNA-signal probe on surface of the modified electrode. Differential pulse voltammetry was employed to record the electrochemical response of biosensor in pH 6.0 solution. As a result, a sensitive oxidation current with a peak potential at 0.10 V (vs. SCE) was obtained corresponding to Cu NPs. The experimental conditions were optimized. Under optimal conditions, the biosensor exhibited wide linear response range (0.5 fM to 70 nM) and low limit of detection (0.03 fM; at S/N = 3). The assay possesses high selectivity and can discriminate analyte microRNA from single-base mismatched microRNA.

Graphical abstract

A sensitive electrochemical biosensor is described for the determination of microRNA-222 by using a dsDNA-templated Cu NPs as signalling probe. (A) represents the preparation of signal probe, and (B) represents the fabrication of electrochemical microRNA sensor.

Keywords

MicroRNA-222 DNA concatemers Copper nanoparticles Biosensor Differential pulse voltammetry 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 20675002).

Supplementary material

604_2019_4011_MOESM1_ESM.docx (654 kb)
ESM 1 (DOCX 654 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and Materials Science, Anhui Key Laboratory of Chem-BiosensingAnhui Normal UniversityWuhuPeople’s Republic of China

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