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Microchimica Acta

, 186:780 | Cite as

Preconcentration and SERS-based determination of infliximab in blood by using a TNF-α-modified gold-coated copper oxide nanomaterial

  • Saiqa Muneer
  • Godwin A. Ayoko
  • Nazrul Islam
  • Emad L. IzakeEmail author
Original Paper
  • 77 Downloads

Abstract

Infliximab (INF) is a chimeric monoclonal immunoglobulin acting against tumor necrosis factor-alpha (TNF-α). The drug is used for the treatment of chronic autoimmune and inflammatory diseases. A target-specific nanomaterial is presented for the extraction of INF from human plasma along with a label-free surface enhanced Raman spectroscopy (SERS) method for its determination using a handheld device. A gold-coated copper oxide chip was functionalized with TNF-α and used to extract the drug from plasma. INF was recovered from the extractor by lowering the pH value to 2.5. The disulfide bond structure of the drug was then reduced and used for its oriented chemisorption onto a gold-coated copper oxide substrate for SERS measurements using the INF-specific band at 936 cm−1. The working range of the SERS method was between 10−7 and 10−14 M of reduced INF. The relative standard deviation (RSD), between three different measurements was 4.2% (intra-day) and 7.1% (inter-day). The quantification and detection limits of the assay (LOQ, LOD) were 0.01 pM and 1.4 fM respectively. The SERS detection was cross-validated against ELISA where 99% agreement was found between the two methods.

Graphical abstract

Schematic representation of the determination of Infliximab (INF) in blood. A gold coated copper oxide chip was functionalised with tumor necrosis factor (TNF-α) and used to extract INF from blood plasma. The captured INF was released, reduced, chemisorbed onto a second gold-coated copper oxide substrate and screened by surface-enhanced Raman spectroscopy (SERS) using a handheld device.

Keywords

Functionalised nanomaterial Tumor necrosis factor Disulfide bond structure TNF inhibitor Therapeutic drug monitoring 

Notes

Acknowledgements

We thank FLEW Solutions Australia for donating gold coated copper oxide substrates for this work.

Supplementary material

604_2019_3947_MOESM1_ESM.docx (1.6 mb)
ESM 1 (DOCX 1687 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Saiqa Muneer
    • 1
    • 2
  • Godwin A. Ayoko
    • 1
    • 2
  • Nazrul Islam
    • 2
    • 3
  • Emad L. Izake
    • 1
    • 2
    Email author
  1. 1.Molecular Design and Synthesis Discipline, Science and Engineering FacultyQueensland University of TechnologyBrisbaneAustralia
  2. 2.Discipline of Environmental Technologies, Science and Engineering FacultyQueensland University of TechnologyBrisbaneAustralia
  3. 3.School of Clinical Sciences, Faculty of HealthQueensland University of TechnologyBrisbaneAustralia

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