Rapid colorimetric determination of dopamine based on the inhibition of the peroxidase mimicking activity of platinum loaded CoSn(OH)6 nanocubes
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Platinum nanoparticles were loaded on CoSn(OH)6 nanocubes via a co-precipitation method. The material (NCs) is shown to be a viable peroxidase mimic that catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to generate oxidized TMB (oxTMB) with absorption at 652 nm. The formation of the blue color can be observed in <30 s. Thus, a visual and colorimetric assay was worked out for H2O2. It has a detection limit as low as 4.4 μM and works in the 5 to 200 μM concentration range. The method was also used to detect dopamine (DA) which is found to inhibit the enzyme mimicking activity of the NCs. Hence, less blue color is formed in its presence. The respective DA assay has a linear response in the 5.0 to 60 μM concentration range and a 0.76 μM detection limit.
KeywordsSynergistic effect Co-precipitation Antioxidants Inhibition Catalytic mechanism Serum
This work was supported by the National Natural Science Foundation of China (Grant No. 21971152), Scientific Research Foundation of Shandong University of Science and Technology for Recruited Talents (Grant No. 2015RCJJ018, 2017RCJJ040 and 2017RCJJ041), Natural Science Foundation of Shandong Province (Grant No. ZR2018MB002, ZR2018MEE003, ZR2018PEE006 and ZR2017BB008), the Science and Technology Projects for Colleges and Universities in Shandong Province (No. J17KA097) and Innovation Fund of Science & Technology of Graduate Students (SDKDYC180239).
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Conflict of interest
The author(s) declare that they have no competing interests.
- 4.Sumathi C, Raju CV, Muthukumaran P, Wilson J, Ravi G (2016) Au-Pd bimetallic nanoparticles anchored on α-Fe2O3 nonenzymatic hybrid nanoelectrocatalyst for simultaneous electrochemical detection of dopamine and uric acid in the presence of ascorbic acid. J Mater Chem B 4:2561–2569. https://doi.org/10.1039/C6TB00501B CrossRefGoogle Scholar
- 6.Li N, Guo J, Liu B, Yu Y, Cui H, Mao L, Lin Y (2009) Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence. Anal Chim Acta 645:48–55. https://doi.org/10.1016/j.aca.2009.04.050 CrossRefPubMedGoogle Scholar
- 9.Wu T, Hou W, Ma Z, Liu M, Liu X, Zhang Y, Yao S (2019) Colorimetric determination of ascorbic acid and the activity of alkaline phosphatase based on the inhibition of the peroxidase-like activity of citric acid-capped Prussian blue nanocubes. Microchim Acta 186:123. https://doi.org/10.1007/s00604-018-3224-5 CrossRefGoogle Scholar
- 10.Li ZM, Zhang X, Pi T, Bu J, Deng RH, Chi BZ, Zheng XJ (2019) Colorimetric determination of the activity of methyltransferase based on nicking enzyme amplification and the use of gold nanoparticles conjugated to graphene oxide. Microchim Acta 186:8. https://doi.org/10.1007/s00604-019-3690-4 CrossRefGoogle Scholar
- 17.Chen J, Chen Q, Chen J, Qiu H (2016) Magnetic carbon nitride nanocomposites as enhanced peroxidase mimetics for use in colorimetric bioassays, and their application to the determination of H2O2 and glucose. Microchim Acta 183(12):3191–3199. https://doi.org/10.1007/s00604-016-1972-7 CrossRefGoogle Scholar
- 19.Liu QY, Yang YT, Li H, Zhu RR, Shao Q, Yang SG, Xu JJ (2015) NiO nanoparticles modified with 5, 10, 15, 20- tetrakis(4-carboxyl pheyl)-porphyrin: promising peroxidase mimetics for H2O2 and glucose detection. Biosens Bioelectron 64:147–153. https://doi.org/10.1016/j.bios.2014.08.062 CrossRefPubMedGoogle Scholar
- 29.Reanpang P, Themsirimongkon S, Saipanya S, Chailapakul O, Jakmunee J (2015) Cost-effective flow injection amperometric system with metal nanoparticle loaded carbon nanotube modified screen printed carbon electrode for sensitive determination of hydrogen peroxide. Talanta. 144:868–874. https://doi.org/10.1016/j.talanta.2015.07.041 CrossRefPubMedGoogle Scholar