Microchimica Acta

, 186:768 | Cite as

Solid-phase nuclear magnetic resonance immunoassay for the prostate-specific antigen by using protein-coated magnetic nanoparticles

  • Pavel KhramtsovEmail author
  • Maria Kropaneva
  • Maria Bochkova
  • Valeria Timganova
  • Svetlana Zamorina
  • Mikhail Rayev
Original Paper


A solid phase NMR-based sandwich immunoassay for the prostate-specific antigen (PSA) is presented. Carbon-encapsulated iron nanoparticles were functionalized with bovine serum albumin, coupled to monoclonal antibodies, and then used as magnetic labels. A nitrocellulose membrane with 8-μm pores was coated with capture antibodies and subsequently incubated with a serum sample and a suspension of the nanoconjugate. Test strips were placed in a portable homemade NMR relaxometer. Magnetic nanoparticles attached to nitrocellulose decrease the T2 relaxation time of the water protons located inside the pores of the membrane. Thus, T2 is inversely proportional to the concentration of the antigen (PSA) in the sample. The assay can be performed within 4 h. The detection limit is 0.44 ng mL−1. Kallikrein 2, human chorionic gonadotropin, and α-fetoprotein do not interfere.

Graphical abstract

Schematic representation of NMR relaxometry-based sandwich dot blot immunoassay of a prostate-specific antigen (PSA). Magnetic nanoparticles bound to immunosorbent decrease the transverse relaxation times (T2) of the water protons located within the pores of the membrane. RF coil: radiofrequency coil.


Dot-immunoassay Relaxometry Nitrocellulose Relaxivity Dot blot 



This work was supported by the Russian Scientific Foundation (grant No. 17-15-01116). The authors would like to thank Dr. Il’ya Byzov (M.N. Mikheev Institute of Metal Physics) for his helpful advice on NMR relaxometry.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

All the procedures performed in the studies involving human participants were in accordance with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. This research was approved by the Review Board of the Institute of Ecology and Genetics of Microorganisms UB RAS (IRB00010009).

Informed consent

Written informed consent was obtained from the volunteers.

Supplementary material

604_2019_3925_MOESM1_ESM.docx (780 kb)
ESM 1 (DOCX 779 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Ecological Immunology, Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences, branch of PSRC UB RASPerm 614081Russia
  2. 2.Department of Microbiology and Immunology, Biology FacultyPerm State National Research UniversityPerm 614000Russia

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