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Microchimica Acta

, 186:842 | Cite as

Polylysine modified conjugated polymer nanoparticles loaded with the singlet oxygen probe 1,3-diphenylisobenzofuran and the photosensitizer indocyanine green for use in fluorometric sensing and in photodynamic therapy

  • Xiao-hui WangEmail author
  • Yin-xiao Yu
  • Kun Cheng
  • Wei Yang
  • Yuan-an Liu
  • Hong-shang PengEmail author
Original Paper
  • 49 Downloads

Abstract

Conjugated polymer hybrid nanoparticles (NPs) loaded with both indocyanine green (ICG) and 1,3-diphenylisobenzofuran (DPBF) are described. The NPs are dually functional in that ICG acts as the photosensitizer, and DPBF as a probe for singlet oxygen (1O2 probe). The nanoparticle core consists of the energy donating host poly(9,9-dioctylfluorenyl-2,7-diyl)-co-(2,5-p-xylene) (PFP). The polymer is doped with the energy acceptor DPBF. Ratiometric fluorometric detection of singlet oxygen is accomplished by measurement of fluorescence at wavelengths of 415 and 458 nm. In addition, the shell of the positively charged polymeric nanoparticles was modified, via electrostatic interaction, with negatively charged PDT drugs ICG. The integrated nanoparticles of type ICG-DPBF-PFP display effective photodynamic performance under 808-nm laser irradiation. The 1O2 sensing behaviors of samples are evaluated based on the ratiometric fluorescent responses produced by DPBF and PFP. 1O2 can be fluorimetically sensed with a detection limit of 28 μM. The multifunctional nanoprobes exhibit effortless cellular uptake, superior photodynamic activity and a rapid ratiometric response to 1O2.

Graphical abstract

Schematic of a dual-functional nanoplatform for photodynamic therapy (PDT) and singlet oxygen (1O2) feedback. It offers a new strategy for self-monitoring photodynamic ablation. FRET: fluorescence resonance energy transfer. Indocyanine green is attached in the shell of nanoparticles, and 1,3-diphenylisobenzofuran is doped into the energy donating host conjugated polymer.

Keywords

Singlet oxygen detection Photodynamic effects DPBF Ratiometric assay Fluorescent probe 

Notes

Acknowledgements

This work was financially supported from the National Nature Science Foundation of China (Grant No. 61605014, 61775245 and 61821001), and the Fundamental Research Funds for the Central Universities (Grant No. 2018RC18 and 2018RC17).

Supplementary material

604_2019_3924_MOESM1_ESM.docx (408 kb)
ESM 1 (DOCX 408 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Work Safety Intelligent Monitoring, School of Electronic EngineeringBeijing University of Posts and TelecommunicationsBeijingChina
  2. 2.School of ScienceMinzu University of ChinaBeijingChina

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