Microchimica Acta

, 186:753 | Cite as

Preparation of deep eutectic solvent-based hexagonal boron nitride-molecularly imprinted polymer nanoparticles for solid phase extraction of flavonoids

  • Xiaoxia Li
  • Kyung Ho RowEmail author
Original Paper


Hexagonal boron nitride (h-BN) is introduced as a 2D scaffold during the preparation of molecularly imprinted polymers (MIPs). The MIPs were prepared from deep eutectic solvents (DES) or from DES containing h-BN, crosslinking agent (ethylene glycoldimethacrylate), initiator (AIBN), porogen (methanol), and template (quercetin). The recognition site of the monomer is protected by the hydrogen bond of the DES before the MIP is polymerized. The formation of the final MIP was analyzed theoretically using density-functional theory. The nanoparticles were characterized by scanning electron microscopy, nitrogen sorption analysis, thermogravimetry and Fourier transform infrared spectroscopy. The introduction of h-BN resulted in an increase in the surface area of the nanoparticles. They were applied as a solid phase extraction sorbent for the extraction of flavonoids (specifically of quercetin, isorhamnetin and kaempferol) from Ginkgo biloba leaves. Following extraction with ethanol, they were quantified by HPLC. The new sorbent has distinctly improved recognition capability for flavonoids compared to conventional MIP nanoparticles.

Graphical abstract

Deep eutectic solvent (DES)-based molecularly imprinted polymer (MIP) was polymerized on the surface of hexagonal boron nitride (h-BN). The h-BN-MIP nanoparticles were applied as a solid phase extraction sorbent for the specific recognition of flavonoids.


Two-dimensional Scaffold Ternary DES Caffeic acid Monomer Hydrogen bond Recognition site Selective recognition Ginkgo biloba leaves Density-functional theory 



This study was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A2C1010032).

Compliance with ethical standards

Conflict of interest

There are no conflicts to declare.

Supplementary material

604_2019_3885_MOESM1_ESM.docx (2.4 mb)
ESM 1 (DOCX 2421 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry and Chemical EngineeringInha UniversityIncheonSouth Korea

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