Microchimica Acta

, 186:746 | Cite as

In-situ graft-crosslinked gold nanoparticles with high-density surface defects and coated with a polytaurine membrane for the voltammetric determination of dopamine

  • Bo Zhang
  • Jixiang Zhang
  • Meili Qie
  • Xiaoyun Bai
  • Mingfei Pan
  • Guozhen FangEmail author
  • Shuo WangEmail author
Original Paper


Well-dispersed and graft-crosslinked gold nanoparticles (AuNPs) were synthesized by the reduction of tetrachloroaurate with hydrazine at room temperature. The AuNPs possess a high density of surface defects which is due to grafting of n-octanoic acid to polyvinylpyrrolidone. The physical and chemical properties of the resulting AuNPs were characterized by UV-vis, XRD, TEM/HRTEM, SAED, and XPS, respectively. The modified AuNPs were placed on a glassy carbon electrode (GCE) in an electropolymerized taurine layer to obtain a sensitive, selective, stable and rapid electrochemical dopamine sensor. The peak current, typically measured at 0.17 V (vs. SCE), increases linearly in the 1.0 to 120 μM dopamine concentration range, and the limit of detection (at S/N = 3) is 0.16 μM with a sensitivity of 2.94 μA·μM−1·cm−2. The sensor was successfully applied to the determination of dopamine in injections and spiked serum samples. The recoveries from spiked serum samples range from 97.5 to 102.4%, with RSDs ranging between 2.8 and 3.4%.

Graphical abstract

Schematic representation of a glassy carbon electrode modified with in-situ graft-crosslinked gold nanoparticles combined with an electropolymerized polytaurine membrane. The sensor exhibits excellent features towards dopamine determination.


Electrochemical sensor Modified electrode Cyclic voltammetry Low-index crystal facet Controllable morphology 



This work was supported by the National Key Research and Development Program of China (project No. 2017YFC1600803) and the Science and Technology Program of Tianjin, China (project No. 17ZYPTJC00050).

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3884_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1.93 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Nutrition and SafetyTianjin University of Science and TechnologyTianjinChina
  2. 2.Beijing Advanced Innovation Center for Food Nutrition and Human HealthBeijing Technology & Business University (BTBU)BeijingChina

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