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Microchimica Acta

, 186:757 | Cite as

A microfluidic immunosensor for visual detection of foodborne bacteria using immunomagnetic separation, enzymatic catalysis and distance indication

  • Gaozhe Cai
  • Lingyan Zheng
  • Ming Liao
  • Yanbin Li
  • Maohua Wang
  • Ning Liu
  • Jianhan LinEmail author
Original Paper
  • 11 Downloads

Abstract

A disposable visual microfluidic immunosensor is described for the determination of foodborne pathogens using immunomagnetic separation, enzymatic catalysis and distance indication. Specifically, a sensor was designed to detect Salmonella typhimurium as a model pathogen. Magnetic nanoparticles (MNPs) were modified with the anti-Salmonella monoclonal antibodies and then used to enrich S. typhimurium from the sample. This is followed by conjugation to polystyrene microspheres modified with anti-Salmonella polyclonal antibodies and catalase to form the MNP-bacteria-polystyrene-catalase sandwich. The catalase on the complexes catalyzes the decomposition of hydrogen peroxide to produce oxygen after passing a micromixer. The generated oxygen gas increases the pressure in the chip and pushes the indicating red dye solution to travel along the channel towards the unsealed outlet. The travel distance of the red dye can be visually read and related to the amount of S. typhimurium using the calibration scale. The sensor can detect as low as 150 CFU·mL−1 within 2 h.

Graphical abstract

Schematic representation of the distance-based microfluidic immunosensor for visual detection of foodborne bacteria using immunomagnetic nanoparticles for bacteria separation, catalase for decomposition of hydrogen peroxide to form oxygen which causes a pressure increase, and red dyed particles movement for distance indication.

Keywords

Microfluidics Distance readout Pathogens detection Enzymatic catalysis Magnetic nanoparticles Polystyrene microspheres Catalase Hydrogen peroxide Chicken samples 

Notes

Acknowledgments

This study was supported in part National Natural Science Foundation of China (31802219) and Walmart Foundation (SA17031161). The authors would like to thank Walmart Food Safety Collaboration Center for its great support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

604_2019_3883_MOESM1_ESM.docx (5.6 mb)
ESM 1 (DOCX 5711 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural AffairsChina Agricultural UniversityBeijingChina
  2. 2.Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of EducationChina Agricultural UniversityBeijingChina
  3. 3.College of Veterinary MedicineSouth China Agricultural UniversityGuangzhouChina
  4. 4.Department of Biological and Agricultural EngineeringUniversity of ArkansasFayettevilleUSA

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