A strategy for visual optical determination of glucose based on a smartphone device using fluorescent boron-doped carbon nanoparticles as a light-up probe
- 43 Downloads
Boronic acid-doped carbon nanoparticles were prepared and are shown to undergo aggregation induced emission (AIE). The nanoparticle composite is a viable fluorescent probe for glucose determination by using the RGB technique and a smartphone. The structure and the chemical composition of the doped carbon nanoparticles were confirmed by SEM, TEM, FTIR and UV-vis spectroscopy. The combination of 4-carboxyphenylboronic acid with o-phenylenediamine and rhodamine B endowed the hybrid with high fluorescence intensity (quantum yield 46%). Compared with conventional two-step preparation of boronic acid-based fluorescent probes for glucose, the present one step synthesis strategy is simpler and more effective. The addition of glucose causes the formation of covalent bonds between the cis-diols group of glucose molecules and boronic acid moiety. Fluorescent intensity can be quantified using dual wavelengths simultaneously, where both increases, as the target analytes bind to the bronic acid. These variations was monitored by the smartphone camera, and the green channel intensities of the colored images were processed by using the RGB option of a smartphone. The assay works in the 32 μM to 2 mM glucose concentration range and has an 8 μM detection limit. The method was successfully used for the assay of glucose in diluted human serum.
KeywordsGlucose sensing Fluorescence Carbon nanoparticle Bronic acid Point of care Smartphone
This research was supported by the Iran Nanotechnology Initiative Council (Smartphone based Sensors and Biosensors project) and the Research Offices of the University of Kurdistan, Sanandaj-Iran (grant number 4.160231). Abdollah Salimi acknowledges professor T.K. Sham (University of Western Ontario) as a host during his sabbatical and also his participation in discussions.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 2.Zou W-S, Ye C-H, Wang Y-Q, Li W-H, Huang X-H (2018) A hybrid ratiometric probe for glucose detection based on synchronous responses to fluorescence quenching and resonance light scattering enhancement of boronic acid functionalized carbon dots. Sensors Actuators B Chem 271:54–63CrossRefGoogle Scholar
- 15.Hamd-Ghadareh S, Salimi A, Fathi F, Bahrami S (2017) An amplified comparative fluorescence resonance energy transfer immunosensing of CA125 tumor marker and ovarian cancer cells using green and economic carbon dots for bio-applications in labeling, imaging and sensing. Biosens Bioelectron 96:308–316CrossRefGoogle Scholar
- 16.Naaz S, Poddar S, Bayen SP, Mondal MK, Roy D, Mondal SK, Chowdhury P, Saha SK (2018) Tenfold enhancement of fluorescence quantum yield of water soluble silver nanoclusters for nano-molar level glucose sensing and precise determination of blood glucose level. Sensors Actuators B Chem 255:332–340CrossRefGoogle Scholar