Microchimica Acta

, 186:754 | Cite as

A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles

  • Cuiyan Wu
  • Lijun Zhu
  • Qiujun Lu
  • Haitao Li
  • Youyu ZhangEmail author
  • Shouzhuo Yao
Original Paper


The authors describe a dual-signal colorimetric and ratiometric fluorescent probe for uric acid (UA). It is based on cascade catalysis and an inner filter effect. The method involves uricase-catalyzed oxidation of UA and iodide-catalyzed oxidation of the colorless peroxidase substrate o-phenylenediamine (OPD) to form yellow 2,3-diaminophenazine (oxOPD). This can be visually observed or monitored by measuring absorbance at 417 nm. Furthermore, oxOPD quenches the fluorescence of silicon nanoparticles (SiNPs) (with peaks at 450 and 565 nm) via an inner filter effect. The change in the ratio of emissions peaking 565 and 450 (at excitation wavelength of 380 nm) increases linearly in the 0.01–0.8 mM UA concentration range). The lower detection limits are 8.4 and 0.75 μM when using the colorimetric and ratiometric fluorometric method, respectively. The assay was successfully applied to the quantitation of UA in spiked serum samples.

Graphical abstract

A dual-signal colorimetric and ratiometric fluor ometric assay was developed for uric acid (UA). The fluorometric assay is based on the inner filter effect between fluorescent silicon nanoparticles and 2,3-diaminophenazine. It involves uricase-catalyzed oxidation of UA, and iodide-catalyzed oxidation of o-phenylenediamine.


Inner filter effect Cascade catalysis Dual-signal detection Colorimetric assay Ratiometric fluorometry Silicon nanoparticles Iodide-catalyzed oxidation o-Phenylenediamine 2,3-Diaminophenazine Human serum analysis 



This work was supported by the National Natural Science Foundation of China (21874042 and 21675051), and the Foundation of the Science & Technology Department of Hunan Province (2016SK2020).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

604_2019_3862_MOESM1_ESM.doc (2.9 mb)
ESM 1 (DOC 3020 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Cuiyan Wu
    • 1
  • Lijun Zhu
    • 1
  • Qiujun Lu
    • 1
  • Haitao Li
    • 1
  • Youyu Zhang
    • 1
    Email author
  • Shouzhuo Yao
    • 1
  1. 1.Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical EngineeringHunan Normal UniversityChangshaPeople’s Republic of China

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