Microchimica Acta

, 186:735 | Cite as

C3N4 nanosheet-supported Prussian Blue nanoparticles as a peroxidase mimic: colorimetric enzymatic determination of lactate

  • Dandan Zhou
  • Congsen Wang
  • Junjun Luo
  • Minghui YangEmail author
Original Paper


Prussian Blue nanoparticles were deposited on g-C3N4 nanosheets. The resulting nanocomposite possesses peroxidase-like (POx) activity and can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine at room temperature in the presence of H2O2. This leads to formation of a blue product with an absorption maximum at 650 nm. The formation of the Prussian Blue nanoparticles on the g-C3N4 nanosheets, and the POx-like activity of the composite were characterized in detail. The POx mimic was used for determination of L-lactic acid via detection of H2O2 that is produced by the enzyme lactate oxidase (LOx). The assay has a linear range that extends from 5 to 100 μM, and the detection limit is 2.2 μM. The method was successfully applied to the determination of L-lactic acid in spiked human serum.

Graphical abstract

Ultra-small Prussian Blue (PB) nanoparticles were used to modify g-C3N4 nanosheets, and their peroxidase-like activity was explored for detection of L-lactic acid. LOx represent L-lactate oxidase, and TMB represents 3,3′,5,5′-tetramethylbenzidine.


g-C3N4 nanosheet Prussian Blue Enzyme mimic Colorimetry L-lactate oxidase 



The authors thank the support of this work by the National Natural Science Foundation of China (Grant No.21575165).

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests. All experiments were in accordance with the guidelines of the National Institute of Health, China, and approved by the Institutional Ethical Committee (IEC) of the Second Xiangya hospital that attached to Central South University. We also obtained informed consent for any experimentation with human serum samples.

Supplementary material

604_2019_3834_MOESM1_ESM.doc (142 kb)
ESM 1 (DOC 142 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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