A gold electrode modified with a gold-graphene oxide nanocomposite for non-enzymatic sensing of glucose at near-neutral pH values
- 118 Downloads
A nanocomposite was prepared from gold and graphene oxide via one-step electrodeposition and used to modify the surface of a gold electrode (Au-Gr/GE) that was then applied to non-enzymatic determination of glucose. The effects of deposition time and supporting substrate on the morphology, structure, and electrochemical properties of the nanocomposite were optimized. The morphologies and crystal structures were characterized by scanning electron microscopy, transmission electron microscopy, and X-ray diffraction. The results indicate that gold nanoparticles grew on the surface of two-dimensional graphene oxide. The electrocatalytic activity of the modified electrode towards glucose oxidation was evaluated by cyclic voltammetry and amperometric methods at pH 7.4. The Au-Gr/GE, typically operated at a potential of 0.00 V (vs. Ag/AgCl), has a linear response in the 0.05–14 mM and 14–42 mM glucose concentration range, high sensitivity (604 and 267 μA cm−2 mM−1) and a low detection limit (12 μM). The modified GE was applied to quantify glucose in sweat where it exhibited excellent sensitivity and accuracy.
KeywordsGlucose detection Non-invasive One-step electrodeposition Sweat High sensitivity Selectivity
The authors acknowledge the financial support from the NSFC (Nos. 21575035, 51572073, 51672074, 11774082), the NSF of Hubei Province (Nos. 2015CFA119, 2016AAA031), Wuhan application foundation frontier Project (No. 2018010401011287), and the Key Lab. of Tobacco Chemistry Foundation of Yunnan Province (No. 2016539200340109).
Compliance with ethical standards
Conflict of interest
The authors certify that there is no conflict of interest with any individual/organization for the present work.
- 1.Nantaphol S, Watanabe T, Nomura N, Siangproh W, Chailapakul O, Einaga Y (2017) Bimetallic Pt-Au nanocatalysts electrochemically deposited on boron-doped diamond electrodes for nonenzymatic glucose detection. Biosens Bioelectron 98:76–82. https://doi.org/10.1016/j.bios.2017.06.034 CrossRefPubMedGoogle Scholar
- 2.Li J, Yuan R, Chai Y, Che X, Li W, Zhong X (2010) Nonenzymatic glucose sensor based on a glassy carbon electrode modified with chains of platinum hollow nanoparticles and porous gold nanoparticles in a chitosan membrane. Microchim Acta 172(1–2):163–169. https://doi.org/10.1007/s00604-010-0485-z CrossRefGoogle Scholar
- 7.Lu LM, Zhang L, Qu FL, Lu HX, Zhang XB, Wu ZS, Huan SY, Wang QA, Shen GL, Yu RQ (2009) A nano-Ni based ultrasensitive nonenzymatic electrochemical sensor for glucose: enhancing sensitivity through a nanowire array strategy. Biosens Bioelectron 25(1):218–223. https://doi.org/10.1016/j.bios.2009.06.041 CrossRefPubMedGoogle Scholar
- 9.Dhara K, Mahapatra DR (2017) Electrochemical nonenzymatic sensing of glucose using advanced nanomaterials. Microchim Acta 185(1):49. https://doi.org/10.1007/s00604-017-2609-1
- 13.Yang J, Yu JH, Rudi Strickler J, Chang WJ, Gunasekaran S (2013) Nickel nanoparticle-chitosan-reduced graphene oxide-modified screen-printed electrodes for enzyme-free glucose sensing in portable microfluidic devices. Biosens Bioelectron 47:530–538. https://doi.org/10.1016/j.bios.2013.03.051 CrossRefPubMedGoogle Scholar
- 21.Olarte O, Chilo J, Pelegri-Sebastia J, Barbe K Glucose detection in human sweat using an electronic nose. In: Engineering in Medicine and Biology Society, 2013. pp 1462–1465Google Scholar
- 30.Chen XM, Lin ZJ, Chen DJ, Jia TT, Cai ZM, Wang XR, Chen X, Chen GN, Oyama M (2010) Nonenzymatic amperometric sensing of glucose by using palladium nanoparticles supported on functional carbon nanotubes. Biosens Bioelectron 25(7):1803–1808. https://doi.org/10.1016/j.bios.2009.12.035 CrossRefPubMedGoogle Scholar