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Microchimica Acta

, 186:672 | Cite as

1-Pyrene carboxylic acid functionalized carbon nanotube-gold nanoparticle nanocomposite for electrochemical sensing of dopamine and uric acid

  • Biyas Posha
  • Haritha Kuttoth
  • Neelakandapillai SandhyaraniEmail author
Original Paper

Abstract

A highly sensitive, selective and cost effective method is described for sensing dopamine (DA) and uric acid (UA). A glassy carbon electrode (GCE) was modified with a nanocomposite consisting of gold nanoparticle-loaded multi-walled carbon nanotube (CNT) modified with 1-pyrene carboxylic acid (PCA). The stable aqueous dispersion of non-covalently functionalized CNT-PCA is an efficient bioprobe for the ultra sensitive and selective detection of dopamine and uric acid in the presence of the potentially interfering agent ascorbic acid (AA). The presence of PCA on the CNT introduces anionic carboxyl groups which repel ascorbate. The presence of the pyrene group augments high electrocatalytic activity towards oxidation of DA and UA, and the gold nanoparticles contribute to the amplification of the signal. The modified GCE gives an excellent peak current with well distinguishable peaks for AA, DA and UA (near −0.08 V, +0.14 V, and +0.22 V vs Ag/AgCl) in differential pulse voltammetry. Chronoamperometric detection of DA (working potential of 0.16 V vs Ag/AgCl) and UA (working potential of 0.3 V vs Ag/AgCl) showed linear ranges of 1 nM-150 μM (LOD 1 nM) and 1 μM–240 μM (LOD 1 μM) for DA and UA, respectively. The nanoprobe was validated by monitoring the recovery of spiked DA and UA in human blood serum samples which indicated a recovery within ±2%.

Graphical abstract

A glassy carbon electrode modified with a gold nanoparticle-loaded multi-walled carbon nanotube (CNT) - 1-pyrene carboxylic acid (PCA) composite was used for the sensitive and selective detection of the dopamine and uric acid.

Keywords

Electrochemical biosensor Nanoprobe Ascorbic acid Neuro transmitter Electrochemical detection Selectivity Real sample analysis Interference 

Notes

Acknowledgements

We thank Kerala State Council for Science, Environment and Technology (KSCSTE) and Department of Science and Technology (DST), India for the financial support to Nano Science Research Laboratory. B.P acknowledges University Grant Commission for the research fellowship.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3783_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1.27 mb)

References

  1. 1.
    Martin C (1998) The Parkinson’s puzzle. Chem Br 34:40–42Google Scholar
  2. 2.
    Wightman RM, May LJ, Michael AC (1988) Detection of dopamine dynamics in the brain. Anal Chem 60:769A–793ACrossRefPubMedGoogle Scholar
  3. 3.
    Heinig M, Johnson RJ (2006) Role of uric acid in hypertension, renal disease, and metabolic syndrome. Cleve Clin J Med 73:1059–1064CrossRefPubMedGoogle Scholar
  4. 4.
    Chih Y-K, Yang M-C (2013) An 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)-immobilized electrode for the simultaneous detection of dopamine and uric acid in the presence of ascorbic acid. Bioelectrochemistry 91:44–51CrossRefPubMedGoogle Scholar
  5. 5.
    Ghanbari K, Moloudi M (2016) Flower-like ZnO decorated polyaniline/reduced graphene oxide nanocomposites for simultaneous determination of dopamine and uric acid. Anal Biochem 512:91–102CrossRefPubMedGoogle Scholar
  6. 6.
    Tavakolia E, Tashkhourian J (2018) Sonication-assisted preparation of a nanocomposite consisting of reduced graphene oxide and CdSe quantum dots, and its application to simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid. Microchim Acta 185:456–464CrossRefGoogle Scholar
  7. 7.
    Liu M, Chen Q, Lai C (2013) A double signal amplification platform for ultrasensitive and simultaneous detection of ascorbic acid, dopamine, uric acid and acetaminophen based on a nanocomposite of ferrocene thiolate stabilized Fe3O4@Au nanoparticles with graphene sheet. Biosens Bioelectron 48:75–81CrossRefPubMedGoogle Scholar
  8. 8.
    Tang C, Tian G, Wang Y, Su Z, Li C, Lin B, Huang H, Yu X, Li X, Long Y, Zeng Y (2009) Selective response of dopamine in the presence of ascorbic acid and uric acid at gold nanoparticles and multi-walled carbon nanotubes grafted with ethylene diamine tetraacetic acid modified electrode. B Chem Soc Ethiopia 23(3):317–326Google Scholar
  9. 9.
    Hou Y, Sheng K, Lu Y, Ma C, Liu W, Men X, Xu L, Yin S, Dong B, Bai X, Song H (2018) Three-dimensional graphene oxide foams loaded with AuPd alloy: a sensitive electrochemical sensor for dopamine. Microchim Acta 185:395–404CrossRefGoogle Scholar
  10. 10.
    Zhao P, Chen C, Ni M (2019) Electrochemical dopamine sensor based on the use of a thermosensitive polymer and an nanocomposite prepared from multiwalled carbon nanotubes and graphene oxide. Microchim Acta 186:134–143CrossRefGoogle Scholar
  11. 11.
    Wang Z, Guo H, Gui R (2018) Simultaneous and selective measurement of dopamine and uric acid using glassy carbon electrodes modified with a complex of gold nanoparticles and multiwall carbon nanotubes. Sensors Actuators B Chem 255:2069–2077CrossRefGoogle Scholar
  12. 12.
    Patrice FT, Qiu K, Zhao L-J Individual Modified Carbon Nanotube Collision for Electrocatalytic Oxidation of Hydrazine in Aqueous Solution. ACS Appl Nano Mater 1:2069–2075Google Scholar
  13. 13.
    Huang B, Liu J, Lai L, Yu F, Ying X, Ye B-C, Li Y (2017) A free-standing electrochemical sensor based on graphene foam-carbon nanotube composite coupled with gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid. J Electroanal Chem 801:129–134CrossRefGoogle Scholar
  14. 14.
    Liu J, Xie Y, Wang K, Zeng Q, Liu R, Liu X (2017) A nanocomposite consisting of carbon nanotubes and gold nanoparticles in an amphiphilic copolymer for voltammetric determination of dopamine, paracetamol and uric acid. Microchim Acta 184:1739–1745CrossRefGoogle Scholar
  15. 15.
    Lingyan J, Xia G, Lisha W, Qi W, Zhichun C, Xianfu L (2013) Electrochemical activation of polyethyleneimine-wrapped carbon nanotubes/in situ formed gold nanoparticles functionalised nanocomposite sensor for high sensitive and selective determination of dopamine. J Electroanal Chem 692:1–8CrossRefGoogle Scholar
  16. 16.
    Jia D, Dai J, Yuan H, Lei L, Xiao D (2011) Selective detection of dopamine in the presence of uric acid using a gold nanoparticles-poly(luminol) hybrid film and multi-walled carbon nanotubes with incorporated β-cyclodextrin modified glassy carbon electrode. Talanta 5:2344–2351CrossRefGoogle Scholar
  17. 17.
    Sun C-L, Chang C-T, Lee H-H (2011) Microwave-assisted synthesis of a core–shell MWCNT/GONR heterostructure for the electrochemical detection of ascorbic acid, dopamine, and uric acid. ACS Nano 5:7788–7795CrossRefPubMedGoogle Scholar
  18. 18.
    Xu H, Zeng L, Xing S (2008) Microwave-radiated synthesis of gold nanoparticles/carbon nanotubes composites and its application to voltammetric detection of trace mercury(II). Electrochem Commun 10:1839–1843CrossRefGoogle Scholar
  19. 19.
    Wang S, Wang X, Jiang SP (2008) PtRu nanoparticles supported on 1-aminopyrene-functionalized multiwalled carbon nanotubes and their electrocatalytic activity for methanol oxidation. Langmuir 24:10505–10512CrossRefPubMedGoogle Scholar
  20. 20.
    Ou Y-Y, Huang MH (2006) High-density assembly of gold nanoparticles on multiwalled carbon nanotubes using 1-pyrenemethylamine as interlinker. J Phys Chem B 110:2031–2036CrossRefPubMedGoogle Scholar
  21. 21.
    Georgakilas V, Tzitzios V, Gournis D, Petridis D (2005) Attachment of magnetic nanoparticles on carbon nanotubes and their soluble derivatives. Chem Mater 17:1613–1617CrossRefGoogle Scholar
  22. 22.
    Saha K, Agasti SS, Kim C (2012) Gold nanoparticles in chemical and biological sensing. Chem Rev 112:2739–2779CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Huang Q, Zhang H, Hu S (2014) A sensitive and reliable dopamine biosensor was developed based on the Au@carbon dots–chitosan composite film. Biosens Bioelectron 52:277–280CrossRefPubMedGoogle Scholar
  24. 24.
    Chen D, Tian C, Li X (2018) Electrochemical determination of dopamine using a glassy carbon electrode modified with a nanocomposite consisting of nanoporous platinum-yttrium and graphene. Microchim Acta 185:98–105CrossRefGoogle Scholar
  25. 25.
    How GTS, Pandikumar A, Ming HN, Ngee LH (2014) Highly exposed {001} facets of titanium dioxide modified with reduced graphene oxide for dopamine sensing. Sci Rep 2014(4):5044Google Scholar
  26. 26.
    Guo Q, Wu T, Liu L (2018) Flexible and conductive titanium carbide–carbon nanofibers for the simultaneous determination of ascorbic acid, dopamine and uric acid. J Mater Chem B 6:4610–4617CrossRefGoogle Scholar
  27. 27.
    Zhang C, Ren J, Zhou J (2018) Facile fabrication of a 3,4,9,10-perylene tetracarboxylic acid functionalized graphene–multiwalled carbon nanotube–gold nanoparticle nanocomposite for highly sensitive and selective electrochemical detection of dopamine. Analyst 143:3075–3084CrossRefPubMedGoogle Scholar
  28. 28.
    Kokulnathan T, Anthuvan AJ, Chen S-M (2018) Trace level electrochemical determination of the neurotransmitter dopamine in biological samples based on iron oxide nanoparticle decorated graphene sheets. Inorg Chem Front 5:705–718CrossRefGoogle Scholar
  29. 29.
    Aparna TK, Sivasubramanian R, Dar MA (2018) One-pot synthesis of Au-Cu2O/rGO nanocomposite based electrochemical sensor for selective and simultaneous detection of dopamine and uric acid. J Alloys Compd 741:1130–1141CrossRefGoogle Scholar
  30. 30.
    Asif M, Aziz A, Wang Z, Wang W, Ajmal M, Xiao F, Chen X, Liu H (2019) Superlattice stacking by hybridizing layered double hydroxide nanosheets with layers of reduced graphene oxide for electrochemical simultaneous determination of dopamine, uric acid and ascorbic acid. Microchim Acta 186:61–72CrossRefGoogle Scholar
  31. 31.
    Li Y, Jiang Y, Song Y (2018) Simultaneous determination of dopamine and uric acid in the presence of ascorbic acid using a gold electrode modified with carboxylated graphene and silver nanocube functionalized polydopamine nanospheres. Microchim Acta 185:382–391CrossRefGoogle Scholar
  32. 32.
    Huang H, Yue Y, Chen Z, Chen Y, Wu S, Liao J, Liu S, Wen H (2019) Electrochemical sensor based on a nanocomposite prepared from TmPO4 and graphene oxide for simultaneous voltammetric detection of ascorbic acid, dopamine and uric acid. Microchim Acta 186:189–198CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Nanoscience Research laboratory, School of Materials Science and EngineeringNational Institute of TechnologyCalicutIndia

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