Microchimica Acta

, 186:655 | Cite as

Colorimetric determination of fumonisin B1 based on the aggregation of cysteamine-functionalized gold nanoparticles induced by a product of its hydrolysis

  • Thaksinan Chotchuang
  • Wilairat Cheewasedtham
  • Titilope John Jayeoye
  • Thitima RujiralaiEmail author
Original Paper


A colorimetric method was developed for the determination of the mold toxin fumonisin B1 (FB1). It is based on the aggregation of cysteamine-capped gold nanoparticles (Cys-AuNPs). The assay involves alkaline hydrolysis of FB1 to obtain hydrolyzed fumonisin B1 (HFB1). The latter induces the aggregation of Cys-AuNPs which results in a color change from wine-red to blue-gray, best at a pH value of 9.0. A plot of absorbance ratio at 645/520 nm versus FB1 concentration is linear in the 2–8 μg kg−1 FB1 concentration range, and the detection limit is 0.90 μg kg−1. Inter-day and intra-day precisions are <6.2%, and recoveries from spiked samples ranged from 93 to 99%. The assay was successfully applied to the determination of FB1 in corn samples. It has a high selectivity over other competitive mycotoxins including aflatoxin, zearalenone, citrinin and patulin. The method is more selective than the detection of FB1 directly which may lead to false-positive errors.

Graphical abstract

Schematic representation of colorimetric assay of fumonisin B1 (FB1). FB1 was alkali-hydrolyzed and its product (hydrolyzed fumonisin B1) induces cysteamine-capped gold nanoparticles (Cys-AuNPs) via hydrogen bondings. The aggregation of Cys-AuNPs causes changes in color from wine-red to blue-gray.


Mycotoxin Noble metals Corn UV-Vis SPE 



gold nanoparticles


cysteamine functionalized gold nanoparticles


fumonisin B1


hydrolyzed fumonisin B1



The authors appreciate the financial support from the Faculty of Science under the Research Assistantship (Contract No. 1-2559-02-002) and partial support from the Department of Chemistry; the Graduate School, and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation, Prince of Songkla University.

Compliance with ethical standards

Conflict of interest

The author declares that there are no conflicts of interest.

Supplementary material

604_2019_3778_MOESM1_ESM.doc (630 kb)
ESM 1 (DOC 629 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of SciencePrince of Songkla UniversitySongkhlaThailand
  2. 2.Analytical Chemistry and Environment Research Unit, Division of Chemistry, Department of Science, Faculty of Science and TechnologyPrince of Songkla UniversityPattaniThailand

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