Colorimetric determination of fumonisin B1 based on the aggregation of cysteamine-functionalized gold nanoparticles induced by a product of its hydrolysis
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A colorimetric method was developed for the determination of the mold toxin fumonisin B1 (FB1). It is based on the aggregation of cysteamine-capped gold nanoparticles (Cys-AuNPs). The assay involves alkaline hydrolysis of FB1 to obtain hydrolyzed fumonisin B1 (HFB1). The latter induces the aggregation of Cys-AuNPs which results in a color change from wine-red to blue-gray, best at a pH value of 9.0. A plot of absorbance ratio at 645/520 nm versus FB1 concentration is linear in the 2–8 μg kg−1 FB1 concentration range, and the detection limit is 0.90 μg kg−1. Inter-day and intra-day precisions are <6.2%, and recoveries from spiked samples ranged from 93 to 99%. The assay was successfully applied to the determination of FB1 in corn samples. It has a high selectivity over other competitive mycotoxins including aflatoxin, zearalenone, citrinin and patulin. The method is more selective than the detection of FB1 directly which may lead to false-positive errors.
KeywordsMycotoxin Noble metals Corn UV-Vis SPE
cysteamine functionalized gold nanoparticles
hydrolyzed fumonisin B1
The authors appreciate the financial support from the Faculty of Science under the Research Assistantship (Contract No. 1-2559-02-002) and partial support from the Department of Chemistry; the Graduate School, and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation, Prince of Songkla University.
Compliance with ethical standards
Conflict of interest
The author declares that there are no conflicts of interest.
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