Microchimica Acta

, 186:658 | Cite as

Sputtering enhanced peroxidase like activity of a dendritic nanochip for amperometric determination of hydrogen peroxide in blood samples

  • Buddhadev Purohit
  • Kuldeep Mahato
  • Ashutosh Kumar
  • Pranjal ChandraEmail author
Original Paper


A nonenzymatic electrochemical nanoprobe is described for the fast determination of hydrogen peroxide (H2O2). A sputtered indium tin oxide electrode with a nano-hierarchical 3D gold structure is used. The nanoprobe was characterized by SEM, EDX, TEM, SAED, and electrochemical techniques. Figures of merit include (a) a fast response time (≤ 1.0 s), (b) two linear dynamic ranges that extend from 10−12 M to 10−10 M and from 10−10 M to10−5 M; and (c) a low limit of detection of 9.8 × 10−13 M. The nanoprobe works in the clinical range and was applied for trace analysis of H2O2 in spiked blood samples, and recoveries ranged between 90 and 96%. It has negligible response (p < 0.001, for n = 3) toward glucose, citric acid, ascorbic acid, uric acid, glycine, and alanine. The shelf-lifetime is found to be 12 weeks.

Graphical abstract

Schematic representation of a dendritic nanochip with peroxidase-like activity. It is made from an indium tin oxide electrode with a nanohierarchical gold structure and was used for amperometric determination of hydrogen peroxide.


Nanoprobe Nanozyme Ultrafast H2O2 detection Indium tin oxide Free radicals Nano engineering 



This work is supported by DST Ramanujan Fellowship (SB/S2/RJN-042/2015) awarded to Dr. Pranjal Chandra. BP, KM, and AK acknowledge the Ph.D. fellowship from IIT, Guwahati. The authors acknowledge the instrumentation facility provided by CIF, IIT Guwahati.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3773_MOESM1_ESM.docx (376 kb)
ESM 1 (DOCX 376 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Buddhadev Purohit
    • 1
  • Kuldeep Mahato
    • 1
  • Ashutosh Kumar
    • 1
  • Pranjal Chandra
    • 1
    Email author
  1. 1.Laboratory of Bio-Physio Sensors and Nano-bioengineering, Department of Bioscience and BioengineeringIndian Institute of Technology GuwahatiGuwahatiIndia

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