Microchimica Acta

, 186:660 | Cite as

Ultrafine Fe3C nanoparticles embedded in N-doped graphitic carbon sheets for simultaneous determination of ascorbic acid, dopamine, uric acid and xanthine

  • Yao Chen
  • Xiao-Fang Zhang
  • Ai-Jun Wang
  • Qian-Li Zhang
  • Hong HuangEmail author
  • Jiu-Ju FengEmail author
Original Paper


A pyrolytic method is described for preparation of ultrafine Fe3C nanoparticles incorporated into N-doped graphitic carbon nanosheets (Fe3C@NGCSs). Iron phthalocyanine and graphitic carbon nitride (g-C3N4) are used as starting materials. The hybrid nanocomposite was placed on a glassy carbon electrode (GCE) and then applied to simultaneous determination of ascorbic acid (AA), dopamine (DA), uric acid (UA) and xanthine (XA). Figures of merits are as follows: for AA, the linear response range covers the 54.0–5491.0 μM range, the lower detection limit is 16.7 μM, and the best working voltage (vs. the saturated calomel electrode (SCE)) is 0.05 V. The respective data for DA are 1.2–120.8 μM, 0.34 μM and 0.19 V (vs. SCE). For UA, the respective data are 4.8–263.0 μM, 1.4 μM and 0.32 V (vs. SCE), and for XA the data are 4.8–361.0 μM, 1.5 μM and 0.71 V (vs. SCE). The method was successfully applied to their simultaneous determination in spiked serum samples.

Graphical abstract

Ultrafine Fe3C nanoparticles embedded in N-doped graphitic carbon sheets for simultaneous determination of ascorbic acid, dopamine, uric acid and xanthine.


Triferrous carbide nanoparticles Graphitic carbon nitride Electrochemical detection Biological small molecule 



This work was financially supported by Zhejiang Public Welfare Technology Application Research Project (LGG19B050001 and LGG18E010001) and the National Natural Science Foundation of China (21475118).

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2019_3769_MOESM1_ESM.doc (4.8 mb)
ESM 1 (DOC 4925 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal UniversityJinhuaChina
  2. 2.School of Chemistry and Biological EngineeringSuzhou University of Science and TechnologySuzhouChina
  3. 3.College of Biological, Chemical Sciences and EngineeringJiaxing UniversityJiaxingChina

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