Microchimica Acta

, 186:641 | Cite as

A nanocomposite prepared from reduced graphene oxide, gold nanoparticles and poly(2-amino-5-mercapto-1,3,4-thiadiazole) for use in an electrochemical sensor for doxorubicin

  • Mohammad Hossein Ghanbari
  • Faezeh Shahdost-Fard
  • Hamid Salehzadeh
  • Mohammad Reza Ganjali
  • Maryam Iman
  • Mehdi Rahimi-NasrabadiEmail author
  • Farhad AhmadiEmail author
Original Paper


A nanocomposite was prepared with reduced graphene oxide, gold nanoparticles and an electropolymerized film made from 2-amino-5-mercapto-1,3,4-thiadiazole. An electrochemical sensor for doxorubicin (DOX) was constructed by modifying a glassy carbon electrode (GCE) with the nanocomposite. The modified GCE was studied by electrochemical techniques which showed it to enable highly sensitive sensing of DOX. Response (typically measured at a typical working potential of −0.56 V vs. Ag/AgCl) is linear in the 30 pM to 30 nM and 30 nM to 30 μM DOX concentration ranges, with a limit of detection (LOD) of 9 pM (at an S/N ratio of 3). The method was applied to the determination of DOX in serum and gave recoveries that ranged between 92 and 108%.

Graphical abstract

A combination of materials consisting of reduced graphene oxide (rGO), gold nanoparticles (AuNPs) and an electropolymerized film of 2-amino-5-mercapto-1,3,4-thiadiazole (poly-AMT, PAMT) is described. The nanocomposite was placed on a glassy carbon elkectrode (GCE) in order to fabricate an electrochemical sensor for doxorubicin (DOX).


Reduced graphene oxide Gold nanoparticles Poly 2-amino-5-mercapto-1,3,4-thiadiazole Doxorubicin Electrochemical sensor 



The financial support of this work by Iran University of Medical Sciences is gratefully acknowledged.

Compliance with ethical standards

The author(s) declare that they have been complianced all ethical standards.

Supplementary material

604_2019_3761_MOESM1_ESM.docx (1022 kb)
ESM 1 (DOCX 954 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Chemical Injuries Research Center, Systems Biology and Poisonings InstituteBaqiyatallah University of Medical SciencesTehranIran
  2. 2.Faculty of PharmacyBaqiyatallah University of Medical SciencesTehranIran
  3. 3.Faculty of ChemistryKharazmi UniversityTehranIran
  4. 4.Department of ChemistryUniversity of IlamIlamIran
  5. 5.Faculty of MedicineIlam University of Medical SciencesIlamIran
  6. 6.Center of Excellence in ElectrochemistryUniversity of TehranTehranIran
  7. 7.Biosensor Research Centre, Endocrinology & Metabolism Molecular and Cellular Research InstituteTehran University of Medical SciencesTehranIran
  8. 8.Physiology Research CenterIran University of Medical SciencesTehranIran
  9. 9.Department of Medicinal Chemistry, School of Pharmacy-International CampusIran University of Medical SciencesTehranIran

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