Microchimica Acta

, 186:644 | Cite as

A nanocomposite prepared from silver nanoparticles and carbon dots with peroxidase mimicking activity for colorimetric and SERS-based determination of uric acid

  • Ailin Wang
  • Ce Guan
  • Guiye ShanEmail author
  • Yanwei Chen
  • Chunliang Wang
  • Yichun Liu
Original Paper


Silver-carbon dots (Ag-CDs) nanocomposites with excellent peroxidase-like and surface-enhanced Raman scattering (SERS) activities were fabricated by reducing silver ion with carbon dots. The formation of the core-shell structure was demonstrated by transmission electron microscopy. The Ag-CD nanocomposite catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to form oxidized TMB (oxTMB) that has a blue color with an absorption maximum at 652 nm. The catalytic activity originates from the fact that the electrons of CDs are transferred to H2O2 and decompose H2O2 into hydroxy radicals. The nanocomposites can be used for uric acid (UA) detection because UA can reduce oxTMB to form colorless TMB. The absorbance drops as the concentration of UA increases from 1 to 500 μM. The SERS signal of oxTMB can be detected (at 1605 cm−1) using the Ag-CD nanocomposites as SERS substrate. The intensity of the SERS signal decreases when the concentration of UA ranges from 0.01 to 500 μM.

Graphical abstract

Schematic representation of the fabrication of silver-carbon dots (Ag-CDs). The Ag-CDs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 to form blue-colored oxidized TMB (oxTMB). UA reduces oxTMB to form colorless TMB. This process is monitored by surface-enhanced Raman scattering (SERS) spectra for UA detection.


Artificial nanozyme Core-shell nanoparticles Optical electric field Catalysis Electron transfer Hydroxy radicals Hydrogen peroxide 3,3',5,5'-Tetramethylbenzidine 



This work was supported by the National Natural Science Foundation of China (11774048, 11374046), and the Project from Key Laboratory for UV-Emitting Materials and Technology of Ministry of Education (No. 130028723).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3759_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1914 kb)


  1. 1.
    Chen YJ, Cao HY, Shi WB, Liu H, Huang Y (2013) Fe-co bimetallic alloy nanoparticles as a highly active peroxidase mimetic and its application in biosensing. Chem Commun 49:5013–5015CrossRefGoogle Scholar
  2. 2.
    Chen HY, Qiu QM, Sharif S, Ying S, Wang Y, Ying Y (2018) Solution-phase synthesis of platinum nanoparticle-decorated metal-organic framework hybrid nanomaterials as biomimetic Nanoenzymes for biosensing applications. Acs Appl Mater Inter 10:24108–24115CrossRefGoogle Scholar
  3. 3.
    Zhang JW, Zhang HT, Du ZY et al (2014) Water-stable metal-organic frameworks with intrinsic peroxidase-like catalytic activity as a colorimetric biosensing platform. Chem Commun 50:1092–1094CrossRefGoogle Scholar
  4. 4.
    Sun AQ, Mu L, Hu XG (2017) Graphene oxide quantum dots as novel Nanozymes for alcohol intoxication. Acs Appl Mater Inter 9:12241–12252CrossRefGoogle Scholar
  5. 5.
    Wang H, Li S, Si YM, Sun Z, Li S, Lin Y (2014) Recyclable enzyme mimic of cubic Fe3O4 nanoparticles loaded on graphene oxide-dispersed carbon nanotubes with enhanced peroxidase-like catalysis and electrocatalysis. J Mater Chem B 2:4442–4448CrossRefGoogle Scholar
  6. 6.
    Datta A, Kapri S, Bhattacharyya S (2016) Carbon dots with tunable concentrations of trapped anti-oxidant as an efficient metal-free catalyst for electrochemical water oxidation. J Mater Chem A 4:14614–14624CrossRefGoogle Scholar
  7. 7.
    Safavi A, Sedaghati F, Shahbaazi H, Farjami E (2012) Facile approach to the synthesis of carbon nanodots and their peroxidase mimetic function in azo dyes degradation. RSC Adv 2:7367–7370CrossRefGoogle Scholar
  8. 8.
    Cailotto S, Mazzaro R, Enrichi F, Vomiero A, Selva M, Cattaruzza E, Cristofori D, Amadio E, Perosa A (2018) Design of Carbon Dots for metal-free Photoredox catalysis. Acs Appl Mater Inter 10:40560–40567CrossRefGoogle Scholar
  9. 9.
    Zhang HC, Ming H, Lian SY, Huang H, Li H, Zhang L, Liu Y, Kang Z, Lee ST (2011) Fe2O3/carbon quantum dots complex photocatalysts and their enhanced photocatalytic activity under visible light. Dalton T 40:10822–10825CrossRefGoogle Scholar
  10. 10.
    Yu H, Zhang HC, Huang H, Liu Y, Li H, Ming H, Kang Z (2012) ZnO/carbon quantum dots nanocomposites: one-step fabrication and superior photocatalytic ability for toxic gas degradation under visible light at room temperature. New J Chem 36:1031–1035CrossRefGoogle Scholar
  11. 11.
    Liu RH, Huang H, Li HT, Liu Y, Zhong J, Li Y, Zhang S, Kang Z (2014) Metal nanoparticle/carbon quantum dot composite as a Photocatalyst for high-efficiency cyclohexane oxidation. ACS Catal 4:328–336CrossRefGoogle Scholar
  12. 12.
    Luo PH, Li C, Shi GQ (2012) Synthesis of gold@carbon dots composite nanoparticles for surface enhanced Raman scattering. Phys Chem Chem Phys 14:7360–7366CrossRefGoogle Scholar
  13. 13.
    Zhao HY, Guo Y, Zhu SJ, Song Y, Jin J, Ji W, Song W, Zhao B, Yang B, Ozaki Y (2017) Facile synthesis of silver nanoparticles/carbon dots for a charge transfer study and peroxidase-like catalytic monitoring by surface-enhanced Raman scattering. Appl Surf Sci 410:42–50CrossRefGoogle Scholar
  14. 14.
    Zhang Y, Xing CS, Jiang DL, Chen M (2013) Facile synthesis of core-shell-satellite ag/C/ag nanocomposites using carbon nanodots as reductant and their SERS properties. Crystengcomm 15:6305–6310CrossRefGoogle Scholar
  15. 15.
    Chen C, Li Y, Kerman S, Neutens P, Willems K, Cornelissen S, Lagae L, Stakenborg T, van Dorpe P (2018) High spatial resolution nanoslit SERS for single-molecule nucleobase sensing. Nat Commun 9:1733CrossRefGoogle Scholar
  16. 16.
    Dies H, Nosrati R, Raveendran J, Escobedo C, Docoslis A (2018) SERS-from-scratch: an electric field-guided nanoparticle assembly method for cleanroom-free and low-cost preparation of surface-enhanced Raman scattering substrates. Colloid Surface A 553:695–702CrossRefGoogle Scholar
  17. 17.
    Shi RY, Liu XJ, Ying YB (2018) Facing challenges in real-life application of surface-enhanced Raman scattering: design and nanofabrication of surface-enhanced Raman scattering substrates for rapid field test of food contaminants. J Agr Food Chem 66:6525–6543CrossRefGoogle Scholar
  18. 18.
    Cheng Z, Choi N, Wang R, Lee S, Moon KC, Yoon SY, Chen L, Choo J (2017) Simultaneous detection of dual prostate specific antigens using surface-enhanced Raman scattering-based immunoassay for accurate diagnosis of prostate cancer. ACS Nano 11:4926–4933CrossRefGoogle Scholar
  19. 19.
    Yang F, Sun P, Zhao HS et al (2018) Genetic association and functional analysis of rs7903456 in FAM35A gene and hyperuricemia: a population based study. Sci Rep-Uk 8:9579CrossRefGoogle Scholar
  20. 20.
    Choe JY, Kim SK (2015) Association between serum uric acid and inflammation in rheumatoid arthritis: perspective on lowering serum uric acid of leflunomide. Clin Chim Acta 438:29–34CrossRefGoogle Scholar
  21. 21.
    Chen HW, Chen YC, Yang FM et al (2018) Mediators of the effects of gender on uric acid nephrolithiasis: a novel application of structural equation modeling. Sci Rep-Uk 8Google Scholar
  22. 22.
    Pan YD, Yang YF, Pang YJ, Shi Y, Long Y, Zheng H (2018) Enhancing the peroxidase-like activity of ficin via heme binding and colorimetric detection for uric acid. Talanta 185:433–438CrossRefGoogle Scholar
  23. 23.
    Kim MC, Kwak J, Lee SY (2016) Sensing of uric acid via cascade catalysis of uricase and a biomimetic catalyst. Sensor Actuat B-Chem 232:744–749CrossRefGoogle Scholar
  24. 24.
    Zhuang QQ, Lin ZH, Jiang YC, Deng HH, He SB, Su LT, Shi XQ, Chen W (2017) Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection. Int J Nanomedicine 12:3295–3302CrossRefGoogle Scholar
  25. 25.
    Ding H, Yu SB, Wei JS, Xiong HM (2016) Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism. ACS Nano 10:484–491CrossRefGoogle Scholar
  26. 26.
    Lee PC, Meisel D (1982) Adsorption and surface-enhanced Raman of dyes on silver and gold sols. J Phys Chem 86:3391–3395CrossRefGoogle Scholar
  27. 27.
    Gao LZ, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583CrossRefGoogle Scholar
  28. 28.
    Nie H, Li MJ, Li QS, Liang S, Tan Y, Sheng L, Shi W, Zhang SXA (2014) Carbon dots with continuously tunable full-color emission and their application in Ratiometric pH sensing. Chem Mater 26:3104–3112CrossRefGoogle Scholar
  29. 29.
    Chen J, Wei JS, Zhang P, Niu XQ, Zhao W, Zhu ZY, Ding H, Xiong HM (2017) Red-emissive carbon dots for fingerprints detection by spray method: coffee ring effect and unquenched fluorescence in drying process. Acs Appl Mater Inter 9:18429–18433CrossRefGoogle Scholar
  30. 30.
    Chen JH, Pang S, He LL, Nugen SR (2016) Highly sensitive and selective detection of nitrite ions using Fe3O4@SiO2/au magnetic nanoparticles by surface-enhanced Raman spectroscopy. Biosens Bioelectron 85:726–733CrossRefGoogle Scholar
  31. 31.
    Li JF, Huang YF, Ding Y, Yang ZL, Li SB, Zhou XS, Fan FR, Zhang W, Zhou ZY, Wu DY, Ren B, Wang ZL, Tian ZQ (2010) Shell-isolated nanoparticle-enhanced Raman spectroscopy. Nature 464:392–395CrossRefGoogle Scholar
  32. 32.
    Ma X, Wen S, Xue X, Guo Y, Jin J, Song W, Zhao B (2018) Controllable synthesis of SERS-active magnetic metal-organic framework-based Nanocatalysts and their application in Photoinduced enhanced catalytic oxidation. ACS Appl Mater Interfaces 10:25726–25736CrossRefGoogle Scholar
  33. 33.
    He YF, Qi F, Niu XH, Zhang W, Zhang X, Pan J (2018) Uricase-free on-demand colorimetric biosensing of uric acid enabled by integrated CoP nanosheet arrays as a monolithic peroxidase mimic. Anal Chim Acta 1021:113–120CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Ailin Wang
    • 1
  • Ce Guan
    • 1
  • Guiye Shan
    • 1
    • 2
    Email author
  • Yanwei Chen
    • 1
  • Chunliang Wang
    • 1
  • Yichun Liu
    • 1
  1. 1.Centre for Advanced Optoelectronic Functional Materials Research, Key Laboratory for UV Light-Emitting Materials and Technology of the Ministry of EducationNortheast Normal UniversityChangchunPeople’s Republic of China
  2. 2.National Demonstration Center for Experimental Physics EducationNortheast Normal UniversityChangchunPeople’s Republic of China

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