Microchimica Acta

, 186:650 | Cite as

Ambient temperature fabrication of a covalent organic framework from 1,3,5-triformylphloroglucinol and 1,4-phenylenediamine as a coating for use in open-tubular capillary electrochromatography of drugs and amino acids

  • Xuan Wang
  • Xiaoyu Hu
  • Yutong Shao
  • Lin Peng
  • Qiqi Zhang
  • Tianhui Zhou
  • Yuhong XiangEmail author
  • Nengsheng YeEmail author
Original Paper


A covalent organic framework (COF) named TpPa-1 was designed and synthesized at ambient temperature by an ultrasound-assisted method from 1,3,5-triformylphloroglucinol (Tp) and 1,4-phenylenediamine (Pa-1). It was utilized as a stationary phase in open-tubular capillary electrochromatography (OT-CEC). The column was coated with TpPa-1 using a covalent bonding strategy. The coated capillary was characterized by morphology, crystallography, and mesoporous analysis to confirm the successful fabrication. The OT-CEC method was utilized for the analysis of tetracyclines, sulfonamides, cephalosporins and amino acids with high-resolution (Rs > 1.81) and good precision (RSD < 4.9%). It takes about 12 h from COF preparation to OT-CEC separation.

Graphical abstract

A covalent organic framework (COF) named TpPa-1 was synthesized at ambient temperature by an ultrasound-assisted method from 1,3,5-triformylphloroglucinol (Tp) and 1,4-phenylenediamine (Pa-1). COF-TpPa-1 modified capillary column was utilized for the analysis of tetracyclines, sulfonamides, cephalosporins and amino acids with high-resolution and good precision.


Ambient temperature fabrication Amino acids Cephalosporins Covalent bonding strategy Covalent organic frameworks Open-tubular capillary electrochromatography Sulfonamides Tetracyclines TpPa-1 



This work was financially supported by the Beijing Natural Science Foundation (2162008).

Authors’ contributions

X. Wang and X. Hu contribute equally to this work.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3741_MOESM1_ESM.pdf (4.6 mb)
ESM 1 (PDF 4.56 MB)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of ChemistryCapital Normal UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food PoisoningBeijing Center for Disease Prevention and ControlBeijingPeople’s Republic of China

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