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Microchimica Acta

, 186:635 | Cite as

A dual-channel ratiometric fluorescent probe for determination of the activity of tyrosinase using nitrogen-doped graphene quantum dots and dopamine-modified CdTe quantum dots

  • Zhengyi Qu
  • Tian Yu
  • Lihua BiEmail author
Original Paper
  • 170 Downloads

Abstract

A dual-channel ratiometric fluorometric assay is described for the determination of the activity of the enzyme tyrosinase (TYR). It is making use of blue-emitting nitrogen-doped graphene quantum dots (bQDs) and of red-emitting dopamine-modified CdTe quantum dots (DA-rQDs). A mixture of the two kinds of quantum dots was prepared, with the ratiometric fluorescence intensity of red to blue adjusted to 5:1. The dopamine on the rQDs is catalytically oxidized by TYR to form dopamine quinone, and this leads to a reduction of the intensity of red fluorescence (peaking at 644 nm). The emission of the bQDs (peaking at 440 nm) represents a stable reference signal. After adding different activities of TYR, the color of the fluorescence of the system continuously changes from red to blue. This can also be visually observed. The ratio of luminescence intensities (at 644/440 nm) can be used to quantify the activity of TYR, and the detection limit is 4.5 mU mL−1. In addition, a test strip is described based on the above method.

Graphical abstract

Schematic representation of the ratiometric fluorometric method for determination of the activity of tyrosinase (TYR). (The full name of the abbreviation in the Scheme: 1-ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC), dopamine (DA), N-hydroxysuccinimide (NHS), nitrogen-doped graphene quantum dots (bQDs), CdTe quantum dots (rQDs)).

Keywords

Enzymatic assay Non-fluorescent substrate Visual detection Colorimetry Test papers 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (21173102 and 21473072).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3733_MOESM1_ESM.docx (860 kb)
ESM 1 (DOCX 856 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of ChemistryJilin UniversityChangchunPeople’s Republic of China
  2. 2.College of Electrical and Electronic EngineeringChangchun University of TechnologyChangchunPeople’s Republic of China

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