Fluorometric determination of nitrite through its catalytic effect on the oxidation of iodide and subsequent etching of gold nanoclusters by free iodine
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A method for sensitive detection of nitrite is presented. It is found that the red fluorescence of gold nanoclusters (with excitation/emission maxima at 365/635 nm) is quenched by traces of iodine via etching. Free iodide is formed by oxidation of iodide by bromate anion under the catalytic effect of nitrite. This catalytic process provides a sensitive means for nitrite detection. Under the optimal conditions, fluorescence linearly dropos in the 10 nM to 0.8 μM nitrite concentration range. The limit of detection is 1.1 nM. This is a few orders of magnitude lower than the maximum concentration allowed by authorities.
KeywordsNitrite Gold nanocluster Fluorescence quenching Iodine Water samples Kinetic fluorescence
This work was supported by the National Natural Science Foundation of China (No. 21305100), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201708) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Compliance with ethical standards
The authors declare that they have no competing interests.
- 1.Lundberg JO, Gladwin MT, Ahluwalia A, Benjamin N, Bryan NS, Butler A, Cabrales P, Fago A, Feelisch M, Ford PC, Freeman BA, Frenneaux M, Friedman J, Kelm M, Kevil CG, Kim-Shapiro DB, Kozlov AV, Lancaster JR, Lefer DJ, McColl K, McCurry K, Patel RP, Petersson J, Rassaf T, Reutov VP, Richter-Addo GB, Schechter A, Shiva S, Tsuchiya K, van Faassen EE, Webb AJ, Zuckerbraun BS, Zweier JL, Weitzberg E (2009) Nitrate and nitrite in biology, nutrition and therapeutics. Nat Chem Biol 5:865–869. https://doi.org/10.1038/nchembio.260 CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Bharath G, Madhu R, Chen S, Veeramani V, Mangalaraja D, Ponpandian N (2015) Solvent-free mechanochemical synthesis of graphene oxide and Fe3O4–reduced grapheneoxide nanocomposites for sensitive detection of nitrite. J Mater Chem A 3:15529–15539. https://doi.org/10.1039/c5ta03179f CrossRefGoogle Scholar
- 10.Manoj D, Saravanan R, Santhanalakshmi J, Agarwal S, Gupta VK, Boukherroub R (2018) Towards green synthesis of monodisperse cu nanoparticles: an efficient and high sensitive electrochemical nitrite sensor. Sensors Actuators B Chem 266:873–882. https://doi.org/10.1016/j.snb.2018.03.141 CrossRefGoogle Scholar
- 18.Kermani HA, Hosseini M, Miti A, Dadmehr M, Zuccheri G, Hosseinkhani S, Ganjali MR (2018) A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters. Anal Bioanal Chem 410:4943–4952. https://doi.org/10.1007/s00216-018-1143-2 CrossRefPubMedGoogle Scholar
- 23.Bai LS, Chi ZH (2001) Kinetic spectrophotometric determination of nitrite by the catalytic oxidation of bromocresol purple with potassium bromate Chin. J Anal Chem 29:926–929. https://doi.org/10.3321/j.issn:0253-3820.2001.08.016 CrossRefGoogle Scholar
- 24.Balasubramanian P, Settu R, Chen S, Chen T, Sharmila G (2018) A new electrochemical sensor for highly sensitive and selective detection of nitrite in food samples based on sonochemical synthesized calcium ferrite (CaFe2O4 ) .clusters modified screen printed carbon electrode. J Colloid Interface Sci 524:417–426. https://doi.org/10.1016/j.jcis.2018.04.036 CrossRefPubMedGoogle Scholar