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Microchimica Acta

, 186:636 | Cite as

An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, β-blockers, and nucleotides

  • Shiyuan Zhao
  • Tao YuEmail author
  • Yingxiang DuEmail author
  • Xiaodong Sun
  • Zijie Feng
  • Xiaofei Ma
  • Wen Ding
  • Cheng Chen
Original Paper
  • 120 Downloads

Abstract

The preparation of an organic polymer monolithic column modified with an amino acid ionic liquid and graphene oxide (AAIL-GO) and its application to capillary electrochromatography (CEC) was described. The AAIL tetramethylammonium-L-arginine was bonded to a monolithic column that was previously modified with graphene oxide by using an hydrochloride/N-hydroxysuccinimide coupling reaction. The morphology of a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was examined by scanning electron microscopy. The incorporation of AAIL and graphene oxide was detected by infrared spectroscopy and elemental analysis. The resulting monolithic column produced a strong and stable electroosmotic flow from the anode to the cathode in the pH range from 3 to 9. Compared with a column modified with AAIL or graphene oxide only, the AAIL-GO-modified column has a better separation ability for amino acids, β-blockers, and nucleotides (the resolution of three amino acids: 2.231 and 2.036, β-blockers: 2.779 and 2.470 and nucleotides: 8.345 and 3.321). Molecular modeling was applied to demonstrate the separation mechanism of small molecules which showed a good support for experimental results.

Graphical abstract

Schematic representation of capillary electrochromatography (CEC) systems with an amino acid ionic liquid-graphene oxide modified organic polymer monolithic column as stationary phases for separation of amino acids, β-blockers, and nucleotides.

Keywords

Molecular modeling Splitting mechanism Tetramethylammonium-L-arginine Ionic liquid Small molecule Glycidyl methacrylate Monolithic column Nanoparticles 

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.: BK20141353).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3723_MOESM1_ESM.docx (418 kb)
ESM 1 (DOCX 417 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Shiyuan Zhao
    • 1
    • 2
  • Tao Yu
    • 1
    • 2
    Email author
  • Yingxiang Du
    • 1
    • 2
    Email author
  • Xiaodong Sun
    • 1
    • 2
  • Zijie Feng
    • 1
    • 2
  • Xiaofei Ma
    • 1
    • 2
  • Wen Ding
    • 1
    • 2
  • Cheng Chen
    • 1
    • 2
  1. 1.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education)China Pharmaceutical UniversityNanjingPeople’s Republic of China
  2. 2.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingPeople’s Republic of China

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