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Microchimica Acta

, 186:611 | Cite as

Colorimetric detection of Staphylococcus aureus using gold nanorods labeled with yolk immunoglobulin and urease, magnetic beads, and a phenolphthalein impregnated test paper

  • Bo Pang
  • Yan Zheng
  • Juan Wang
  • Yushen Liu
  • Xiuling Song
  • Jinhua Li
  • Shuo Yao
  • Kaiyue Fu
  • Kun XuEmail author
  • Chao ZhaoEmail author
  • Juan LiEmail author
Original Paper

Abstract

A colorimetric test is described for the rapid detection of Staphylococcus aureus (SA). Gold nanorods (AuNRs) are first labeled with urease and yolk immunoglobulin (IgY). This probe can specifically bind SA. In the next step, nonspecific magnetic beads and sample are added. This leads to the formation of the AuNR-IgY-SA-nMB immunocomplex which is then magnetically separated. Finally, a solution of urea is added to the supernatant. Ureases catalyzes the decomposition of urea which results in an increase in the pH value. The increase in the pH value is detected by using a phenolphthalein test paper which undergoes a color change from white to pink. The analytical process can be completed within 20 min. The method is highly specific and can detect as little as 476 cfu·mL−1. It was verified by analyzing contaminated Chinese cabbage and beef samples, and 1000 cfu·mL−1 of SA were accurately detected.

Graphical abstract

Schematic representation of a colorimetric method for the detection of Staphylococcus aureus based on the immunocomplex formed from dual-labeled gold nanorod (AuNR) probe, bacteria and non-specific magnetic bead (nMB). This method can be completed within 20 min.

Keywords

Food safety Visual detection Nanoparticles Immunocomplex Magnetic separation Urea pH change Image analysis 

Notes

Funding

This work was supported by the National Natural Science Foundation of China (Grant No.: 81872668, 81473018), Jilin Province Science and Technology Development Plan Item (Grant No.: 20170204003SF) and Graduate Innovation Fund of Jilin University (Grant No.: 101832018C061).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3722_MOESM1_ESM.docx (5.1 mb)
ESM 1 (DOCX 5260 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Public HealthJilin UniversityChangchunPeople’s Republic of China
  2. 2.Department of Geriatricsthe First Hospital of Jilin UniversityChangchunPeople’s Republic of China

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