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Microchimica Acta

, 186:628 | Cite as

Ultrasensitive electroluminescence biosensor for a breast cancer marker microRNA based on target cyclic regeneration and multi-labeled magnetized nanoparticles

  • Dongli Chen
  • Meng Zhang
  • Fenyue Zhou
  • Hong HaiEmail author
  • Jianping LiEmail author
Original Paper
  • 39 Downloads

Abstract

An electrochemiluminescent (ECL) biosensor is described for the determination of the breast cancer biomarker microRNA. The method is based on the amplification via target cyclic regeneration through a system of hairpin DNA probes, primers, and Klenow fragment of DNA polymerases combined with CdTe quantum dots (QDs) and gold nanoparticles. The assay is performed by exploiting the luminescence properties of CdTe-QDs and K2S2O8 as a co-reactive agent to increase the ECL signal. It was successfully applied to ECL-based detection of a 20-mer microRNA. The sensor has a linear response in the 0.1 fM to 0.2 pM microRNA concentration range and a detection limit as low as 33 aM. The assay has been applied to the determination of microRNA spiked in serum samples, and recoveries ranged from 94.4 to 100.5%.

Graphical abstract

A novel electroluminescence biosensor based on the amplification of target cyclic regeneration is described. It is achieved by using a system of hairpin DNA probes, primers, and Klenow fragment of DNA polymerases combined with CdTe QDs and Au NPs, and was successfully applied to microRNA detection.

Keywords

Biosensor Electrochemiluminescence Breast cancer MicroRNA Quantum dots Polymerase Enzyme-assistant Signal amplification Cyclic regeneration Serum samples 

Notes

Acknowledgements

The authors gratefully acknowledge the financial support from National Natural Science Foundation of China (No. 21765006), the Natural Science Foundation of Guangxi Province of China (2015GXNSFFA139005; 2018GXNSFAA050023), Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, High Level Innovation Teams of Guangxi Colleges & Universities and Outstanding Scholars Program (Guijiaoren[2014]49) for this research project.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Ethical standards and informed consent

The study and experimental sections were approved by the Hospital of Guilin University of Technology. Human serum samples used in this study do not have any identifying information about all the participants that provided written informed consent.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and BioengineeringGuilin University of TechnologyGuangxiChina
  2. 2.Guangxi Key Laboratory of Electrochemical and Magnetochemical Function MaterialsGuangxiChina

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