An electrochemiluminescence immunosensor for myoglobin using an indium tin oxide glass electrode modified with gold nanoparticles and platinum nanowires
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An electrochemiluminescence (ECL) immunosensor was fabricated to detect myoglobin in human serum. Specifically, gold nanoparticles and platinum nanowires were deposited onto indium tin oxide-coated glass with 3-aminopropyl-trimethoxysilane as the linker to fabricate a basal electrode. The gold nanoparticles had a diameter of approximately 5 nm, and the platinum nanowires had diameters of approximately 2–3 nm and lengths on the order of dozens of nanometers. The nanomaterials effectively enhanced the ECL of luminol and enabled it to emit strong light, even in a weakly basic environment. A myoglobin antibody was then covalently immobilized on the electrode. Upon formation of the immunocomplex, the intensity of the luminol ECL was reduced. Under the optimized experimental conditions, the intensity of the ECL linearly decreased with the logarithm of the myoglobin concentration over the range of 3.0 ng·mL−1 to 0.32 μg·mL−1, and the detection limit was 0.11 ng·mL−1.
KeywordsElectrochemiluminescence Immunosensor Nanocomposite Inhibition Myoglobin Acute myocardial infarction
This work is financially supported by the National Natural Science Foundation of China (21675115, 21375091).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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