Microchimica Acta

, 186:518 | Cite as

Copper(II)-coated Fe3O4 nanoparticles as an efficient enzyme mimic for colorimetric detection of hydrogen peroxide

  • Hongying Liu
  • Langlang Zhu
  • Huan Ma
  • Jiajun Wen
  • Hanxiao Xu
  • Yubing Qiu
  • Linan ZhangEmail author
  • Lihua LiEmail author
  • Chunchuan GuEmail author
Original Paper


The authors describe the preparation of Cu(II)-coated Fe3O4) nanoparticles (NPs) that possess excellent peroxidase-like activity. The NPs were formed by chelation between Cu(II) ions and the oxygen functional groups of sodium ligninsulfonate. The morphology and structure of the NPs were characterized by scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The NPs have an average diameter of 220 nm. They are shown to be viable peroxidase mimics that can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide to produce a blue coloration. The findings were used to design a colorimetric assay that has a linear response in the 2.5 to 100 μM H2O2 concentration range and a 0.2 μM detection limit. The assay excels by its selectivity, high sensitivity, good selectivity, portability and cost efficiency.

Graphical abstract

Fe3O4-Cu2+ nanoparticles with excellent peroxidase-like activity were successfully prepared via a facile strategy, and then used to design a facile as well as sensitive colorimetric H2O2 sensor. The linear range and the detection limit were 2.5~100 μM and 0.212 μM.


Fe3O4-Cu2+ nanoparticles Nanozyme Biosensor Hydrogen peroxide Colorimetric method 



This study was financed by the Science and Technology Program of Zhejiang Province of China (LGF18H200005), NSFC (21405029, and 61731008), the Medical and Health Technology Development Program of Zhejiang province (2017KY533), and the Young and Middle-aged Academic Leaders of Zhejiang Province.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3599_MOESM1_ESM.docx (401 kb)
ESM 1 (DOCX 401 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Hangzhou Dianzi UniversityHangzhouChina
  2. 2.Deparment of Clinical LaboratoryHangzhou Cancer HospitalHangzhouChina

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