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Microchimica Acta

, 186:447 | Cite as

Intracellular fluorometric determination of microRNA-21 by using a switch-on nanoprobe composed of carbon nanotubes and gold nanoclusters

  • Yuanyuan Liu
  • Liping Jiang
  • Xiaojian Fan
  • Pingping Liu
  • Shenghao XuEmail author
  • Xiliang LuoEmail author
Original Paper
  • 49 Downloads

Abstract

A sensitive and rapid fluorometric “switch on” assay is described for the detection of microRNA-21. It is based on the use of a fluorescence resonance energy transfer pair consisting of lysozyme-modified gold nanoclusters (Lys-Au NCs) and carbon nanotubes (CNTs). The Lys-Au NCs can be synthesized by a microwave-assisted technique within 2.5 min. They were modified with the ss-DNA probe (a 22-mer) for microRNA-21. Once the ss-DNA associates with the CNTs due to π stacking, the orange-red fluorescence (with excitation/emission peaks at 500/610 nm) is quenched. Nevertheless, the quenched fluorescence can be recovered after addition of microRNA-21 because of the stronger affnity between ss-DNA and microRNA-21. On the basis of the fluorescence recovery at 610 nm caused by microRNA-21, the latter can be quantified in the 0.01 to 100 nM concentration range, with a 36 pM detection limit. The method was applied to the determination of microRNA-21 in spiked serum with recoveries ranging from 98.6% to 110.0%. It also enables normal and cancer cells to be differentiated by direct imaging of intracellular microRNA-21.

Graphical abstract

A sensitive “switch on” FRET-based fluorometric assay for microRNA-21 is described. It is based on the use of  lysozyme-modified gold nanoclusters (Lys-Au NCs) and carbon nanotubes (CNTs) as energy donor and energy acceptor, respectively.

Keywords

Fluorescence resonance energy transfer Cell imaging Fluorescence Hybridization specificity 

Notes

Acknowledgments

The authors gratefully acknowledge the support from the National Nature Science Foundation of China (21505081, 21675093), the Natural Science Foundation of Shandong Province of China (ZR2019YQ13) and the Taishan Scholar Program of Shandong Province, China (ts20110829).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3573_MOESM1_ESM.doc (460 kb)
ESM 1 (DOC 460 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  2. 2.Department of Breast SurgeryThe Eighth Peoples’ Hospital of QingdaoQingdaoChina
  3. 3.Zhengzhou Tobacco Research InstituteCNTCZhengzhouPeople’s Republic of China

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