Microchimica Acta

, 186:475 | Cite as

Rapid identification and antibiotic susceptibility test of pathogens in blood based on magnetic separation and surface-enhanced Raman scattering

  • Jia Li
  • Chongwen WangEmail author
  • Luoluo Shi
  • Liting Shao
  • Peiwen Fu
  • Keli Wang
  • Rui XiaoEmail author
  • Shengqi WangEmail author
  • Bing GuEmail author
Original Paper


An effective surface-enhanced Raman scattering (SERS) method is presented for the rapid identification and drug sensitivity analysis of pathogens in blood. In a first step, polyethyleneimine-modified magnetic microspheres (Fe3O4@PEI) were used to enrich bacteria from blood samples. Next, the Fe3O4@PEI@bacteria complex was cultured on both ordinary and drug-sensitive plates. Lastly, the SERS spectra of single colonies were acquired in order to identify different pathogens and their resistant strains by comparison with established standardized bacterial SERS spectras and orthogonal partial least squares discriminant analysis (OPLS-DA) method. Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and their resistant strains were used to evaluate the performance of the SERS method. The results demonstrate that the method can accurately detect and identify all the tested sensitive and drug-resistant strains of bacteria, including 77 clinical blood infection samples. The method provides a way for rapid identification and susceptibility test of pathogens, and has great potential to replace currently used time-consuming methods.

Graphical abstract

Schematic presentation of a method for the rapid identification and drug sensitivity analysis of pathogens in blood. It is based on a combination of magnetic separation, SERS fingerprint analysis and orthogonal partial least squares discriminant analysis (OPLS-DA).


SERS Raman spectroscopy Orthogonal partial least squares discriminant analysis (OPLS-DA) Antibiotics Cross-validation Fe3O4@PEI Antibiotic-resistant bacteria Identification 



This research was supported by the National Natural Science Foundation of China (No 81471994, 81830101), Jiangsu Provincial Medical Talent (ZDRCA2016053), Six talent peaks project of Jiangsu Province (WSN-135), Advanced health talent of six-one project of Jiangsu Province (LGY2016042), Infectious diseases major project(2018ZX10712001-010).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3571_MOESM1_ESM.doc (1.9 mb)
ESM 1 (DOC 1958 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Medical Technology Institute of Xuzhou Medical UniversityXuzhouPeople’s Republic of China
  2. 2.Department of Laboratory MedicineAffiliated Hospital of Xuzhou Medical UniversityXuzhouPeople’s Republic of China
  3. 3.Beijing Institute of Radiation MedicineBeijingPeople’s Republic of China
  4. 4.College of Life SciencesAnhui Agricultural UniversityHefeiPeople’s Republic of China

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