Microchimica Acta

, 186:430 | Cite as

Photoelectrochemical aptasensing of ofloxacin based on the use of a TiO2 nanotube array co-sensitized with a nanocomposite prepared from polydopamine and Ag2S nanoparticles

  • Xiaofei Qin
  • Liping Geng
  • Qianqian Wang
  • Yan WangEmail author
Original Paper


A photoelectrochemical (PEC) method is described for aptamer-based detection of ofloxacin (OFL). It is making use of a TiO2 nanotube array (NTA) that is sensitized with a structure composed of polydopamine and silver sulfide nanoparticles. The NTA were prepared by a two-step synthetic method. First, the TiO2 nanotube electrode was covered with Ag2S nanoparticles via successive ionic layer adsorption and reaction strategy. Next, they were coated with a thin film of polydopamine (PDA) by in-situ polymerization. The inorganic/organic nanocomposites exhibit distinctly enhanced visible-light PEC activity. This was exploited to fabricate a PEC aptasensor. The PDA film serves as both the sensitizer for charge separation and as a support to bind the aptamer against OFL. The aptasensor undergoes a decrease in photocurrent due to the formation of the aptamer-OFL complex. Under the optimized conditions and at a typical working potential of 0 V (vs. Hg/Hg2Cl2), the NTA has a linear response in the 5.0 pM to 100 nM OFL concentration range and a 0.75 pM detection limit (at S/N = 3). The aptasensor was successfully applied to the determination of OFL in spiked milk samples.

Graphical abstract

Schematic illustration for the preparation and mechanism of the photoelectrochemical aptasensor for ofloxacin. TiO2 NTs: TiO2 nanotube arrays; PDA: polydopamine; MCH: 6-mercapto-1-hexanol; OFL: ofloxacin; PEC: photoelectrochemistry; CB: conduction band; VB: valence band; LUMO: the lowest unoccupied molecular orbital; HOMO: the highest occupied molecular orbital; AA: ascorbic acid.


Inorganic/organic nanocomposite Sensitization Aptasensor Antibiotic detection Food sample 


Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3566_MOESM1_ESM.doc (633 kb)
ESM 1 (DOC 633 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine ChemicalsShandong Normal UniversityJinanPeople’s Republic of China

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