In-situ microscale spectrophotometric determination of phenytoin by using branched gold nanoparticles
- 34 Downloads
A rapid method for the sensitive detection of phenytoin (PHT) by branched gold nanoparticles (B-AuNPs) is described. These nanoparticles were synthesized by adding methanol as the reducing agent and poly(ethylene glycol) as the stabilizer at 70 °C. The B-AuNPs are red in color with an absorption maximum at 540 nm when prepared in situ. However, the color becomes increasingly weaker when PHT is present in increasing concentrations. This method can determine PHT over the 67–670 ng·mL−1 concentration range, with detection limit of 21 ng·mL−1. The relative standard deviation for five replicate measurements at 68 and 530 ng·mL−1 of PHT was 3.2% and 1.2%, respectively. The method was applied to the determination of PHT in plasma samples of epileptic patients, and the results were in agreement with those obtained by a standard official method.
KeywordsDilantin Epilepsy Plasma HAuCl4 Poly(ethylene glycol) Methanol Reducing agent Stabilizing agent Localized surface plasmon resonance Colorimetry
This work was financially supported by the “Vice Chancellor of Research and Technology, Tabriz University of Medical Sciences” under the grant number of 594252.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 7.Serralheiro A, Alves G, Fortuna A, Rocha M, Falcão A (2013) First HPLC–UV method for rapid and simultaneous quantification of phenobarbital, primidone, phenytoin, carbamazepine, carbamazepine-10,11-epoxide, 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine, lamotrigine, oxcarbazepine and licarbazepine in human plasma. J Chromatogr B 925:1–9CrossRefGoogle Scholar
- 13.Mantri MA, Shanmukhappa S (2010) UV spectrophotometric estimation of phenytoin sodium in pure and pharmaceutical formulations. Asian J Chem 22:2447–2449Google Scholar
- 19.Vasimalai N, John SA (2013) Aggregation and de-aggregation of gold nanoparticles induced by polyionic drugs: spectrofluorimetric determination of picogram amounts of protamine and heparin drugs in the presence of 1000-fold concentration of major interferences. J Mater Chem B 1:5620CrossRefGoogle Scholar
- 22.Karami-Bonari AR, Tamizi E, Samini M, Jabbaribar F, Jouyban A (2009) HPLC method for simultaneous determination of five antiepileptic drugs in rat plasma. Asian J Chem 21:6501–6507Google Scholar
- 29.Ingle JD, Crouch SR (1988) Spectrochemical analysis. Prentice Hall, Englewood CliffsGoogle Scholar