A sandwich-type electrochemical aptasensor for the carcinoembryonic antigen via biocatalytic precipitation amplification and by using gold nanoparticle composites
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A sandwich-type electrochemical aptasensor is described for detecting the carcinoembryonic antigen (CEA) with high sensitivity and accuracy. Two kinds of nanomaterials are used. The first was obtained by modifying gold nanoparticles with reduced graphene oxide and hemin (Hemin-rGO-AuNPs). The second consists of horseradish peroxidase-modified organic-inorganic hybrid nanoflowers linked to gold nanoparticles to obtain an architecture of type HRP-Cu3(PO4)2-HNF-AuNPs). These serve as carriers for two aptamers (apt1 and apt2) against CEA. Simultaneously, they were used to catalyze the precipitation reaction between 4-chloro-1-naphthol(4-CN) and H2O2. A sandwich-type assay linked to enzyme inhibition amplification was established for electrochemical determination of CEA. Under optimal experimental conditions and by using differential pulse voltammetry, the response peak currents (best measured at −0.34 V vs. Ag/AgCl) increases linearly with the logarithm of the CEA concentration in the range between 100 fg mL−1 and 100 ng mL−1. The detection limit is as low as 29 fg mL−1.
KeywordsOrganic-inorganic hybrid nanoflowers Horseradish peroxidase Aptamer Peroxidase-like mimic Hybrid materials Hemin 4-Chloro-1-naphthol Signal inhibition amplification Differential pulse voltammetry Tumor biomarker
The authors are sincerely grateful for the financial support from the National Natural Science Foundation of China (Grant no. 21575130; U1504216) and Startup Research Fund of Zhengzhou University (Grant no. 1511316006).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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