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Microchimica Acta

, 186:483 | Cite as

Preparation of graphene oxide quantum dots from waste toner, and their application to a fluorometric DNA hybridization assay

  • Qiang Xu
  • Yan Gong
  • Zhifeng Zhang
  • Yanming Miao
  • Dongxia Li
  • Guiqin YanEmail author
Original Paper
  • 64 Downloads

Abstract

A one-pot hydrothermal method was developed for the synthesis of graphene oxide quantum dots (GOQDs). It is making use of toner waste as the precursor and H2O2 as the oxidant. Synthesis takes 4 h and does not require strong acids or complex purification steps and does not produce environmentally harmful metal ions. The GOQDs display blue fluorescence with excitation/emission maxima at 340/445 nm. The feasibility of detecting specific DNA sequence was promoted using polyethyleneimine to modify the GOQDs surface. A method was developed to recognized a specific DNA sequence. This is based on electrostatic aggregation of GOQDs and ssDNA labeled with Dabcyl at the 3′ end, which promotes fluorescence quenching of GOQDs. The possible fluorescence quenching mechanism (which is mainly dynamic) was investigated using the Stern-Volmer equation. When a target sequence was added, which is complementary to the ssDNA, the dabcyl-labeled ssDNA is released due to strict complementary base pairing. This promotes fluorescence recovery of GOQDs. The assay has a 0.17 nM detection limit and a linear range of 0.5–30 nM. The method was used to quantify specific DNA sequences from extracts of genetically modified plant tissues.

Graphical abstract

Graphene oxide quantum dots (GOQDs) were synthesized by one-pot hydrothermal method using waste toner, and the surface was modified by polyethyleneimine (PEI). Through the interaction of PEI-GOQDs with Dabcyl-DNA single strands to dynamically quench the fluorescence of GOQDs. Based on DNA hybridization technology, we established specific DNA sequence detection nanoprobe.

Keywords

Graphene oxide quantum dots Dynamic quenching Fluorometric assay DNA Transgenic Nanoprobe 

Notes

Acknowledgments

This work was funded by the National Natural Science Foundation of China (NSFC) (Grant 31700862), the Natural Science Foundation for Young Scientists of Shanxi Province (Grant 201601D021109), and Shanxi Province Graduate Education Innovation Program (Grant 2017BY086).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3539_MOESM1_ESM.doc (63.5 mb)
ESM 1 (DOC 64990 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shanxi Normal UniversityLinfenPeople’s Republic of China

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